Comparative Study on Different Modified Techniques Used For DNA Isolation From Teeth Samples for Obtaining Optimized Output

Authors

  • Indresh Kumar Mishra1 , Amarnath Mishra2 , Amit C Kharkwal3 , Braja Kishore Mohapatra4 , Chittaranjan Behera5 , Bhoopendra Singh6

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10374

Keywords:

DNA, RT-PCR, PCR, STR Analysis, Forensic Science

Abstract

DNA isolation is a process used for isolation of DNA from different types of samples using a combination

of physical and chemical methods. A comparative study is made to isolate DNA from 30 teeth samples with

some pre PCR and post PCR modifications in the organic Phenol: Chloroform: isoamyl alcohol, Automate

ExpressTM prepfiler BTA kit and QIAamp® DNA Investigator Kit. Our result showed significant results

which will be helpful in short listing the array of these techniques.

Author Biography

Indresh Kumar Mishra1 , Amarnath Mishra2 , Amit C Kharkwal3 , Braja Kishore Mohapatra4 , Chittaranjan Behera5 , Bhoopendra Singh6

1 Assistant Director, Biology, Forensic Science Laboratory, Rohini, Govt. of NCT of Delhi, Delhi, India, 2Assistant

Professor-II, Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India, 3 Deputy Director,

Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India, 4Principal Scientific

Officer, Biology, Central Forensic Science Laboratory (CBI), CGO Complex, New Delhi, India, 5 Additional

Professor, Department of Forensic Medicine & Toxicology, AIIMS, New Delhi, India, 6Scientific Assistant, Forensic

Science laboratory, Rohini, Govt. of NCT of Delhi, India

Published

2020-07-30

How to Cite

Indresh Kumar Mishra1 , Amarnath Mishra2 , Amit C Kharkwal3 , Braja Kishore Mohapatra4 , Chittaranjan Behera5 , Bhoopendra Singh6. (2020). Comparative Study on Different Modified Techniques Used For DNA Isolation From Teeth Samples for Obtaining Optimized Output. Indian Journal of Forensic Medicine & Toxicology, 14(3), 305-310. https://doi.org/10.37506/ijfmt.v14i3.10374