A Study of Histochemical Characteristics of Mucosubstances in Normal Endocervical Glands

Authors

  • Swapna Shedge A1 , Padmaja. A Havle2 , Megha A. Doshi3, Priya Roy4

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10455

Keywords:

Mucosubstances, Special stains, Cervical mucins ,Normal.

Abstract

Background: The apical epithelial surfaces of mammalian respiratory, gastrointestinal, and reproductive

tracts are coated by mucus, a mixture of water, ions, glycoproteins, proteins, and lipids. Mucins are mainly

of two types- Neutral and Acidic. Mucus plays an important role in reproductive function and defence of

the female reproductive tract. Objectives: To know the mucin distribution in normal endocervical glands.

Methodology: This is a retrospective, observational , analytical, case control study aimed to evaluate mucin

histochemical pattern in normal endocervical glands. Ten histologically proven blocks of normal endocervical

glands were taken. Tissue sections were stained by, PAS, PAS-diastase, Phenylhydrazine-PAS, Alcian blue

pH 2.5, Alcian Blue pH 1, combined Alcian blue-PAS, Aldehyde fuschin and combined Aldehyde fuchsin

Alcian blue techniques. Results: Results were tabulated according to colour intensity into different grades

ranging from + to ++++. Regarding mucin histochemistry of normal endocervical glands; the epithelium

and glands show mixture of both neutral and acidic mucins. Acidic mucins are more in amount than neutral.

In acidic mucins, sialomucins are predominantly seen. Conclusion: Mucin histochemical patterns serve as

valuable, cost-effective tool for diagnosis in histopathology and for the researchers in histology, where a

slight change in the mucin pattern may help in the early diagnosis of the disease process.

Author Biography

Swapna Shedge A1 , Padmaja. A Havle2 , Megha A. Doshi3, Priya Roy4

1Assistant Professor, Department of Anatomy, 2Assistant Professor, Department of Obstretics and Gynaecology,

3Professor and Head, Department of Obstretics and Gynaecology, 4 Assossiate Professor, Department of Anatomy,

KIMS, Deemed To Be University, Karad and Maharashtra

Published

2020-07-30

How to Cite

Swapna Shedge A1 , Padmaja. A Havle2 , Megha A. Doshi3, Priya Roy4. (2020). A Study of Histochemical Characteristics of Mucosubstances in Normal Endocervical Glands. Indian Journal of Forensic Medicine & Toxicology, 14(3), 735-740. https://doi.org/10.37506/ijfmt.v14i3.10455