Association between IL12A Gene of G/A genotype Polymorphism and Pulmonary Tuberculosis Risk in Baghdad Population

Authors

  • Anwar Abed Nasser Dhabaan1 and Mayes Abdul-Hadi Mahdi2

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10501

Keywords:

IL12A Gene, Pulmonary Tuberculosis, Polymorphisms, Tetra-ARMS PCR

Abstract

The study included eighty samples. This cases-controlled study was performed including fifty Pulmonary

tuberculosis patients, their ages ranged from 12 to 77 year (27 female and 23 male) and thirty controls

(healthy), their ages ranged from 19 to 58 year (15 female and 15 male). We confined the frequency of

IL12A gene (G/A rs568408 genotype) polymorphism by Tetra-ARMS PCR (Tetra amplification refractory

mutation system-polymerase chain reaction) technology. Also, we determined the association of IL12A

Gene (G/A rs568408 genotype) polymorphism with Pulmonary tuberculosis patients in Baghdad. Statistical

results showed significant difference in genotype frequency of IL12A Gene (G/A rs568408 genotype)

polymorphism between Pulmonary tuberculosis patients and control (healthy). The G allele shows high

frequency in Pulmonary tuberculosis patients comparison with control (healthy) and present related with

etiological fraction risk of Pulmonary tuberculosis patients and its ratio 62% in patients and 51.67% in

control (healthy), While A allele shows high frequency in control (healthy) comparison with Pulmonary

tuberculosis patients and present related with protective fraction of Pulmonary tuberculosis patients and

its ratio 48.33% in control and 38% in Pulmonary tuberculosis patients. The genotypes of GG and AA

homozygotes shows high frequency in Pulmonary tuberculosis patients comparison with control (healthy),

and its ratio 42% and 18% respectively in Pulmonary tuberculosis patients, while its ratio 3.33% and 0%

respectively in control (healthy), also GG and AA genotypes appear related with etiological fraction risk of

Pulmonary tuberculosis patients, while the GA heterozygote show high frequency in control (healthy) and its

ratio 96%, GA genotypes related with preventive fraction of Pulmonary tuberculosis patients. Our findings

demonstrate that the IL12A Gene (G/A rs568408 genotype) polymorphism may represent a significant risk

factor for pulmonary tuberculosis patients in Baghdad population.

Author Biography

Anwar Abed Nasser Dhabaan1 and Mayes Abdul-Hadi Mahdi2

1 Assit prof Dr., 1Post graduate. AL-Iraqia University/ Collage of Education/department of biology, Baghdad, Iraq

Published

2020-07-30

How to Cite

Anwar Abed Nasser Dhabaan1 and Mayes Abdul-Hadi Mahdi2. (2020). Association between IL12A Gene of G/A genotype Polymorphism and Pulmonary Tuberculosis Risk in Baghdad Population. Indian Journal of Forensic Medicine & Toxicology, 14(3), 990-995. https://doi.org/10.37506/ijfmt.v14i3.10501