Toxopathological and Cytogenetic Effects of Commercial Sweetener Aspartame after Chronic Oral Administration in Rat Pups
Keywords:Aspartame, chronic toxicity, pups, cytogenetics.
Objective: This study was done to evaluate the potential toxic effect of aspartame (APS) artificial sweetener
after chronic oral administration in rat pups as a model for children that consumption high quantities of
different types of sweets and juices from the peddler, regularly and daily.
Methods: Twenty-four rat pups were divided equally into three groups. Therapeutic dose group (T1) and
double dose group(T2) received 0.08, 0.16 g/kg. body weight(BW), respectively, while control group (C)
received distilled water orally for 90 (d)days.
Results: The result of present study revealed that there was an increased consumption of food, water and
arising nervous signs(aggressive) in T1 and T2 groups comparing with control. The cytogenetic study
includes the mitotic index and blast index showing a substantial decline relative to the control one in
both treated groups. While the blast index showed a significant reduction(P<0.05) in T2 relative to T1
and the control group. No chromosomal aberration observed in all groups exposed to aspartame. Different
histopathological lesions were recorded in the liver of T1 group represented by inflammatory cell (neutrophil
& mononuclear cell) aggregation around the blood vessels. While noted in a double dose group (0.16g/
kg.bw), a granulomatous lesion with mild change in fat is shown.
Conclusion: Used daily for lengthy periods ASP has cytogenetic and pathological risks.
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