Gene Expression and Levels of Plasma Protein Tyrosine Phosphatase Non-Receptor Type 22 (PTPN22) in Pulmonary Tuberculosis Patients and Their Household Contacts in Makassar, Indonesia

Authors

  • Ibrahim A1,3, Djaharuddin I2 , Hidayah N1 , Subair1 , Handayani I1 , Tenriola A1 , Massi MN4,5

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10537

Keywords:

PTPN22, Pulmonary tuberculosis, Gene expression, Real time PCR, ELISA, Level of plasma.

Abstract

Background: The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gen,has been involved in

the immune response to tuberculosis infection by affecting the inflammatory response and subsequent anti

microbial immunity. This study aims to determine the PTPN22 expression and differences in the levels of

PTPN22 in pulmonary TB patients (PTB) with household contacts and healthy control.

Methods: We analyzed PTPN22 expression and the level of plasma PTPN22 from pulmonary tuberculosis

patients (PTB), household contacts and healthy control using real time PCR and ELISA method.

Results: ThirtyPTB, 30 household contacts and 30 control were involved in this study. Analysis of the

assocation of PTPN22 gene expression against TB showed that PTPN22 gene expression was 7.3 times

upregulated compared to household contact and 12.1 times upregulated compared to healthy controls.Levels

of plasma PTPN22 in PTB: 10.0620 ng/ml, in Household contact: 6.7923 ng/ml and in control: 4.4293 ng/

  1. These values did not differ significantly between the patients, household contact and control.

Conclusion: Our study results found that PTPN22 gene expression is significantly increased in PTB than

household contact and control.Levels of plasma PTPN22 in PTB patientsdid not differ significantly than in

household contact and control.

Author Biography

Ibrahim A1,3, Djaharuddin I2 , Hidayah N1 , Subair1 , Handayani I1 , Tenriola A1 , Massi MN4,5

1Postgraduate Program, Medical Faculty, Hasanuddin University, Indonesia, 2Pulmonogy Department, Medical Faculty, Hasanuddin University, Indonesia, 3 Community Center for Lung Health Makassar, Indonesia, 4Hasanuddin University Medical Research Center, Medical Faculty, Hasanuddin University, Indonesia, 5 Microbiology Department, Medical Faculty, Hasanuddin University, Indonesia

Published

2020-07-30

How to Cite

Ibrahim A1,3, Djaharuddin I2 , Hidayah N1 , Subair1 , Handayani I1 , Tenriola A1 , Massi MN4,5. (2020). Gene Expression and Levels of Plasma Protein Tyrosine Phosphatase Non-Receptor Type 22 (PTPN22) in Pulmonary Tuberculosis Patients and Their Household Contacts in Makassar, Indonesia. Indian Journal of Forensic Medicine & Toxicology, 14(3), 1180-1185. https://doi.org/10.37506/ijfmt.v14i3.10537