Role of Interleukin-36 in Response to Pseudomonas Aeruginosa Infection

Authors

  • Muslim Idan Mohsin1 , Mohammed Jasim Al-Shamarti1 , Rusul Idan Mohsin3 , Sarmad Al-Sahaf2

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10545

Keywords:

IL36, Pseudomonas aeruginosa, LRT infection and A549 cells

Abstract

Background: One of the causative agents of the lower respiratory tract (LRT) is Pseudomonas aeruginosa

which can lead to severe infection associated with a lung infection. Many cytokines are secreted in response

to bacterial infection, in particular, interleukin IL-36 cytokine in response to P. aeruginosa infection. IL36

promotes primary immune response via binding to the IL-36 receptor (IL-36R). Indeed, an over activity of

IL-36 might be an initiating factor for many immunopathologic sceneries in pneumonia.

The aims of Study: the involvement of IL-36 in the P. aeruginosa infection could be a clue to find a specific

way for treatments of different inflammatory and degenerative lung diseases.

Methodology and Results: we used manual and VITEK2 system to isolate and purify all the species, also

using qPCR real-time to demonstrate that the involvement of IL-36 cytokine in response P. aeruginosa

infection which is isolated from (LRT). We found that one of the most common pathogens is P. aeruginosa

after Klebsiella pneumonia, and more resistance bacterium against antibiotics is P. aeruginosa (P4).

Furthermore, we found that IL-36 cytokine increased in response to P. aeruginosa infection.

Conclusion: P. aeruginosa is one of the main causative agents in LRT in many hospitals in Iraq, and the

contamination and antibiotics resistance for these nosocomial pathogens is increased. Also, IL-36 expression

significantly upregulated in human lung epithelial (A549 cells) after infected by P. aeruginosa at the mRNA

level.

Author Biography

Muslim Idan Mohsin1 , Mohammed Jasim Al-Shamarti1 , Rusul Idan Mohsin3 , Sarmad Al-Sahaf2

1 Department of Laboratory Investigations, Faculty of Science, University of Kufa, Najaf, Iraq. 2Baghdad College

of Dentistry, University of Baghdad. 3 Department pathological analysis, collage of Al-Tossi University, Najaf, Iraq

Published

2020-07-30

How to Cite

Muslim Idan Mohsin1 , Mohammed Jasim Al-Shamarti1 , Rusul Idan Mohsin3 , Sarmad Al-Sahaf2. (2020). Role of Interleukin-36 in Response to Pseudomonas Aeruginosa Infection. Indian Journal of Forensic Medicine & Toxicology, 14(3), 1219-1224. https://doi.org/10.37506/ijfmt.v14i3.10545