Molecular and Phylogenetic Study of Sarcocystis Gigantia from Different Slaughterhouse Regions in Karbala province

Authors

  • Kawther A.M. Al-Mussawi 1, Hayder A. Muhammed 2 , Saja A. M. Al-Saadi 3

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10567

Keywords:

Sarcocystis gigntea, Phylogenetic analysis, PCR, sensitivity and specificity.

Abstract

Sarcocystis diseases caused a serious illness in sheep flock which inducing decrease of growth conversion

rates and complete or partial loss of carcasses at the abattoirs, This article which described an prevalence of

parasites Sarcocystis gigantea in sheep slaughtered in a farm in kerbala governerate, Iraq, and performed a

phylogenetic analysis by using the Euclidean distances calculation from the Sarcosystis gigntea nucleotide

frequencies around the world sarcosystis genomes. Fifty sheep showed multiple nodules in the esophagus

that were microscopically characterized as encapsulated cysts filled with elongated, nucleated structures

morphologically consistent with S. gigantea bradyzoites. The preverlance and species of Sarcocystis gigantia

were revealed as 10% (5 out f 50) for the macroscopic sarcosystis , the shape of these cysts were resembled

to oval and pear shape, the sensitivity of Polymerase chain reaction was 75% with 95% of confidence

intervals 19.41% to 99.37% and the specificity was 95.65% with 95% of confidence intervals 85.16% to

99.47%. Our results suggest that oligonucleotide sequences were useful for estimation of Sarcocystis gigntea

phylogenetic relationships for closely related species.

Author Biography

Kawther A.M. Al-Mussawi 1, Hayder A. Muhammed 2 , Saja A. M. Al-Saadi 3

1 M.Sc. Student, Department of Biology, College of Education For pure Science, University of Kerbala, 2Assist.

Prof., Department of Microbiology, College of Veterinary Medicine, University of Kerbala, 3Assist. Prof., Department

of Biology, College of Education for pure Science, University of Kerbala

Published

2020-07-30

How to Cite

Kawther A.M. Al-Mussawi 1, Hayder A. Muhammed 2 , Saja A. M. Al-Saadi 3. (2020). Molecular and Phylogenetic Study of Sarcocystis Gigantia from Different Slaughterhouse Regions in Karbala province. Indian Journal of Forensic Medicine & Toxicology, 14(3), 1283-1288. https://doi.org/10.37506/ijfmt.v14i3.10567