An in Vitro Study to Evaluate Antibacterial Effect Thymus Vulgaris Essential Oil Against Porphyromonas Gingivalis in Kurdistan– Iraq

Authors

  • Hama Rash A1 , Kareem S2 , Hamaamin H 3 , Qadr K3 , Salih S4, Azeez S2 , Sha A5

DOI:

https://doi.org/10.37506/ijfmt.v14i3.10568

Keywords:

Porphyromonas gingivalis, Thymus vulgaris, minimum inhibitory concentration, periodontitis.

Abstract

Purpose: Porphyromonas gingivalis is a keystone among perio-pathogengenic bacteria to initiate

periodontitis and treatment is better when the periodontal pathogens including Porphyromonas gingivalis

are totally eliminated. The essential oil of Thymus vulgaris has many bioactive constituents having

antimicrobial activity against many bacteria. This study investigates the antibacterial effect of this extracts

against Porphyromonas gingivalis. Methods: Sub gingival plaque samples were collected from individuals

having periodontitis with the probing pocket depth of at least 6mm; Conventional microbiological tests and

molecular technique used for confirmation of Porphyromonas gingivalis, hydrodistillation method was used

for extraction of essential oil. Antibacterial activity of the extract against Porphyromonas gingivalis has been

determined by using two fold serial dilution method to determine minimum inhibitory concentration (MIC)

and minimum bactericidal concentration (MBC). Result: the essential oil extracted from Thymus vulgaris

demonstrated antimicrobial effect against Porphyromonas gingivalis growth. Conclusion:The extract was

able to demonstrate inhibitory and bactericidal activity against Porphyromonas gingivalis. Further studies

should focus on the use of Thymus vulgaris essential oil in the treatment of periodontitis.

Author Biography

Hama Rash A1 , Kareem S2 , Hamaamin H 3 , Qadr K3 , Salih S4, Azeez S2 , Sha A5

1 Lecturer, Protected Agriculture Department, Bakrajo Technical Institute, Sulaimani Polytechnic University,

Sulaimani, Iraq, 2 Lecturer, Dental Nursing Department, Sulimani Technical Institute, Sulaimani Polytechnic

University, Sulaimani, Iraq, 3 Lecturer, Department of Biology, College Of Science / University of Sulaimani,

Sulaimani, Iraq, 4 Assistant Lecturer, Food Science and Quality control department, College of Agriculture

engineering. University of Sulimani, Sulimani, Iraq, 5 Lecturer, Periodontics Department, College of Dentistry,

University of Sulimani, Sulimani, Iraq

Published

2020-07-30

How to Cite

Hama Rash A1 , Kareem S2 , Hamaamin H 3 , Qadr K3 , Salih S4, Azeez S2 , Sha A5. (2020). An in Vitro Study to Evaluate Antibacterial Effect Thymus Vulgaris Essential Oil Against Porphyromonas Gingivalis in Kurdistan– Iraq. Indian Journal of Forensic Medicine & Toxicology, 14(3), 1289-1293. https://doi.org/10.37506/ijfmt.v14i3.10568