Genetic Survey of Enteroaggregative E.coli in Diarrheic Children under 5 years in Thi-qar governorate
Keywords:Enteroaggregative E.coli, virulence genes, antibiotic resistance, ESBL.
The study aimed to investigate the prevalence of Enteroaggregative E.coli obtained from children of Thi-qar
suffered from diarrhea through PCR detection of virulence factors (aggR, astA, pic) antibiotic resistance and
extended spectrum ?-lactamase (ESBL) production. The investigation included collection 430 stool samples
from diarrheic children under five years involved both gender and 40 samples as a control from healthy
children , were consulted in (Bint al- Huda Hospital for Gynecology obstetrics and pediatrics , Al-Mousawi
hospital and private clinics laboratory ) at a period extended from 10th December 2018 -21th July 2019. A
total of the isolates were diagnosed by different laboratory and molecular method. PCR diagnosis confirmed
the distribution of genes (aggR and astA) within EAEC were (66% and 53%), respectively. The highest
resistance to antibiotics was (100%) to ceftazidime and cefpodoxime , (97% ) to ceftriaxone, followed by
(96%) for Ampicillin, Sulfa-Trimethoprim and cefuroxime, respectively, and cefixime, amoxiclav, Cefotaxim
and Tetracycline (94.8%), (92.3%), (85%) and (78.2%), respectively. While the medium resistance was
(55.13%) for each of ciprofloxacin , piperacillin-tazobactam, respectively, and Amikacin (51%). While
the lowest resistance was to Gentamicin (30.7%) and Imipenem (26.9%). EAEC isolates were multidrug
resistance in average 64/66 (96.97%).
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