The Effect of Storage and Time of Blood Specimen Examination of Plasma Prothrombin Time and Activated Partial Thromboplastin Time Stabilities
Keywords:activated partial thromboplastin time, examination time, preanalytic coagulation study, prothrombin time, hematology
his was an analytical study with cross sectional design. Collection of blood specimens was carried out by
consecutive sampling during March-June 2019. Blood specimens in this study were healthy adults aged ?21
years who underwent general medical check-up or blood donors who met the inclusion criteria and signed
informed consent. A total of 71 samples were stored at room temperature and temperature 4-8 °C, then PT
(prothrombin time) and aPTT (activated partial thromboplastin time) checks were carried out at 0, 4, 12, and
24 hours. Data were analyzed by using Kolmogorov-Smirnov Test, paired t test and Wilcoxon Signed Rank
Test. There were differences in the results of PT in the room temperature specimens and 4-8 °C at 4 hours,
12 hours, and 24 hours with p value <0.001. This was also found in the aPTT parameter, but for specimens
at 4-8 °C at the 24th hour examination the results of changes in aPTT were not statistically significant
with p values 0.062. Different PT and aPTT tests at the two storage areas and at different examination
times obtained different and statistically significant results with p value <0.001. Examination of hemostasis
physiology requires special attention, where pre-analytic plays an important role which can influence the
overall test results. The aspects of time and storage on the stability of PT and aPTT specimens are illustrated
in this study where significant differences were obtained with p values <0.001. Examination and storage of
coagulation study specimens in accordance with CLSI recommendations will provide accurate results and
accurately describe the state of the specimen according to the patient’s clinical condition.
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