The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX

Authors

  • Ahmad Yudianto1 , Simon Martin Manyanza Nzilibili2 , Pudji Harjanto2 , Fery Setiawan2

Keywords:

Touch DNA, STR CODIS, Identification

Abstract

Introduction:Identification through DNA analysis is an accurate diagnostic tool. DNA analysis is via

Variable Number of Tandem Repeat (VNTR) and Restriction Fragment Length Polymorphisms (RFLP).

Up to date, blood spots/blood, sperm spots, vaginal swabs, buccal swabs and bones are specimens that

are widely used in the field of forensics for DNA analysis. At the crime scene, the perpetrator’s skin may

accidentally be in contact with surrounding objects thereby transferring trace evidence to the objects.

Method and Materials: Laboratory observation to demonstrate identification through DNA isolation from

the objects that are touched (touch DNA), using the STR CODIS locus, with a momentary research design.

DNA was isolated from buccal swabs and swab properties (watches and mobile phones) from volunteers

who have signed the consent form. A total of ten samples were used in this study.

Results and Discussion: Mean levels of DNA [UV-Visible Spectrophotometer] buccal swab: 167.89 ±

85.71 µg / ml, watch swab: 59.19 ± 5.58 µg / ml, mobile swab: 38.09 ± 2.12 µg / ml and the purity of the

buccal swab DNA: 1.79 ± 0.71, the watch swab: 1.69 ± 0.76, the mobile swab:1,53 ± 0,56. Visualization of

PCR products onPolyacrylamide Agarose Composite Gel Electrophoresis [PAGE] stained with Silver and

amplified using the standard primers THOI, TPOX and CSF1POfor STR CODIS showed a 100% detection

of amplicons. Allele profiles on all samples of STR CODIS were a match or identical to the positive control

K562.

Conclusion:Property (handphone and watch) swabs can be used as alternative materials in forensic

identification using Touch DNA analysis. It was able to be isolated and amplified by using Polymerase Chain

Reaction on the STR CODIS loci THO1, CSF1PO and TPOX

Author Biography

Ahmad Yudianto1 , Simon Martin Manyanza Nzilibili2 , Pudji Harjanto2 , Fery Setiawan2

1 Forensic Science Program, Post Graduate School, 2Postgraduate Student of Forensic Science Program,

Airlangga University, Surabaya

Published

2020-07-30

How to Cite

Ahmad Yudianto1 , Simon Martin Manyanza Nzilibili2 , Pudji Harjanto2 , Fery Setiawan2. (2020). The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX. Indian Journal of Forensic Medicine & Toxicology, 14(3), 1740-1744. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10667