Comparative Evaluation of Microscopy and Loop-Mediated Isothermal Amplification (Lamp) Assay for the Diagnosis of Tuberculosis
Keywords:Tuberculosis, Global health problem, LAMP, Diagnostic tool, Microscopy
Tuberculosis (TB) is one of the major global health problem which affects millions of people each year. For
the routine diagnosis of TB, microscopic technique is used but it has lower sensitivity and specificity. This
study was carried out to evaluate the efficacy of loop mediated isothermal amplification (LAMP) over smear
microscopy to detect Mycobacterium tuberculosis. Out of 84 processed samples processed in this study, the
diagnostic tests showed varying results. Using smear microscopy, 16(19.05%) and with the LAMP assay,
17(20.24%) cases were found positive for M. tuberculosis. The sensitivity of the LAMP assay (87.5%) was
greater than that of microscopy (82.35%) while the specificity of both methods was comparable (>95%).
Being more sensitive than microscopy, LAMP assay is more likely to show the positive result and solve
the errors in diagnosis of the TB cases. Thus, LAMP assay can be an important and cost-effective tool for
appropriate and timely diagnosis of TB patients. This can further assist to implement intervention programs
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