Impact of Exposure Period to Liquid Nitrogen Vapor on Criteria of Human Spermatozoa Cryopreserved in New Cryopreservation Technique

Authors

  • Ahmed H. Zwamel1 , Muhammad-Baqir M-R. Fakhrildin2 , Hayfa H. Hassani3

Keywords:

ART; cryopreservation; human spermatozoa; LN2 vapor;ovarian follicles; sperm cryopreservation,

Abstract

Background: The emptied sheep’s ovarian follicles recently used as a container for spermatozoa during

cryopreservation, it was found a proper carrier to cryopreserving spermatozoa in vapor-dependent

cryopreservation. The aim of this study was to evaluate the effect of two periods of exposure to liquid

nitrogen (LN2)vapor on the parameter of spermatozoa during cryopreservation in this technique.

Method: The study was conducted on 30 semen samples from patients with oligozoospermia diagnosed

by semen analysis according to the standard criteria of World Health Orgnization (WHO) 2010. Sheep’s

ovarian follicles obtained from local slaughterhouse and prepared by slicing the ovaries and evacuating the

follicular fluid and oocyte. Each semen sample diluted 1:1 with cryosolution (glycerol 10%) and injected

within eight emptied sheep’s ovarian follicles. The first four follicles represent P1; exposed to LN2 vapor for

7.5 minutes and the other four follicles represent P2; exposed to LN2 vapor for 15 minutes before emerged

in liquid nitrogen. Sperm progressive motility, total motility, normal morphology and DNA fragmentation

index (DFI) were analyzed for all samples pre-freezing and post-thawing.

Results: After two months of cryopreservation, sperm progressive motility and total motility significantly

(P<0.01) increased post-thawing in P2 as compared with P1, while both of P1 and P2 significantly (P<0.01)

decreased as compared with pre-freezing. Normal morphology significantly (P<0.01) decreased post

thawing in both P1 and P2 as compared with pre-freezing, while no significantly difference foundbetween

P1and P2. DFI significantly (P<0.01) increased post-thawing in P1 and P2 as compared with pre-freezing,

while in P2 DFI was significantly lower than in P1.

Conclusions: The exposure to liquid nitrogen vapor for 15 minutes in emptied ovarian follicles technique

gives a better results than exposure to the vapor for 7.5 minutes regarding sperm progressive motility, total

motility and DFI.

Author Biography

Ahmed H. Zwamel1 , Muhammad-Baqir M-R. Fakhrildin2 , Hayfa H. Hassani3

1 Lecturer,Department of Medical Physiology, Faculty of Medicine, Jabir Ibn Hayyan Medical University,

Najaf, Iraq. “PhD Student, Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq”,

2Professor, Department of Medical Physiology, Faculty of Medicine, Jabir Ibn Hayyan Medical University, Najaf,

Iraq, 3 Professor, Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

Published

2020-07-30

How to Cite

Ahmed H. Zwamel1 , Muhammad-Baqir M-R. Fakhrildin2 , Hayfa H. Hassani3. (2020). Impact of Exposure Period to Liquid Nitrogen Vapor on Criteria of Human Spermatozoa Cryopreserved in New Cryopreservation Technique. Indian Journal of Forensic Medicine & Toxicology, 14(3), 2379-2384. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10792