Investigation of the Hydrolytic Enzyme Activities of Candida Parapsilosis Isolated From Milk Samples of Bovine Mastitis by API ZYM and Molecular Method

Authors

  • Hawraa F.H. AL-abedi1 , Azhar A.F. AL-Attraqchi2 , Bassam Y. Khudaie3

Keywords:

Candida parapsilosis, Bovine mastitis, Hydrolytic enzymes, Conventional PCR, API ZYM test.

Abstract

Two hundred and fifty milk samples were collected from cows suffering from mastitis, these samples were

tested for mycotic mastitis using culture and conventional PCR method. The results showed 116 isolates of

Candida spp. were recovered with a prevalence of (46.4%),hence C. parapsilosis was the dominating species

15/116 (12.9%). Fifteen isolates (100%) belong C.parapsilosis was confirmed by PCR using a species

specific primer for the 18S rRNA gene. Some randomly chose isolates of C. parapsilosis were used for

detection of their hydrolytic enzymatic activity by API ZYM test and PCR. The method used to evaluate their

enzymatic activity was API ZYM (BioMerieux). The highest activity was observed for leucine arylamidase,

acid phosphatase, naphthol-AS-BI-phosphohydrolase and ?-glucosidase as 10/10 (100%) for each, followed

by esterase lipase (C8) 9/10 (90%), valine arylamidase and alkaline phosphatase 8/10 (80%) for both and

esterase (C4) 7/10 (70%). Conventional PCR was done for partial amplification of sterol esterase, alkaline

phosphates and alpha-glucosidase genes by specific primer sequences, The results of the PCR amplification

of these genes was 8/10 (80%) for sterol esterase gene. Concerning the alkaline phosphatase gene, it was

present in 10/10 (100%). Regarding alpha-glucosidase gene was present in 10/10 (100%). This work aims at

evaluating enzymatic activities of C. parapsilosis isolated from cow’s milk with mastitis.

Author Biography

Hawraa F.H. AL-abedi1 , Azhar A.F. AL-Attraqchi2 , Bassam Y. Khudaie3

1 Lecturer Dr. Department of Microbiology, PhD student in College of Veterinary Medicine/the University of

Basrah/Iraq, 2 Assist Prof. Dr. Department of Medical Microbiology/Collage of Medicine, Al-Nahrain University/

Iraq, 3Prof. Dr. Department of Microbiology/College of Veterinary Medicine, University of Basrah/Iraq

Published

2020-07-30

How to Cite

Hawraa F.H. AL-abedi1 , Azhar A.F. AL-Attraqchi2 , Bassam Y. Khudaie3. (2020). Investigation of the Hydrolytic Enzyme Activities of Candida Parapsilosis Isolated From Milk Samples of Bovine Mastitis by API ZYM and Molecular Method. Indian Journal of Forensic Medicine & Toxicology, 14(3), 2343-2349. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10803