In situ Molecular Hybridization of Kaposi, s Sarcoma Associated Virus (Human Herpes Virus 8) in Nasopharyngeal Carcinoma Tissues

Authors

  • Zainab A. Hamid1 , Zahraa Ali Abdullah2 , Isra’a Mahdi Al-Sudani3 , Saad Hasan Mohammed Ali4, Shakir H. Mohammed Al-Alwany5 , Khalil Ismail A. Mohammed

Keywords:

HHV-8; Nasopharyngeal carcinoma; Inflammatory nasal polyps; CISH.

Abstract

Background: Viral, dietary and genetic factors are implicated in the etiology ofnasopharyngeal cancer, a rare

type of head and neck cancers. Unlike other viruses, HHV-8 encodes several human cytokines homologues

and regulatory genes that play important roles in the viral pathogenesis.

Objective: To analyze the rates of HHV-8 infection in tissues obtained from a group of patients with

nasopharyngeal carcinoma and inflammatory nasal polyps (INP) .

Patients and Method: One hundred- thirty formalin-fixed, paraffin- embedded nasopharyngeal carcinoma

and nasal inflammatory polyps tissues enrolled in this study; 65 nasopharyngeal tissue biopsies from

nasopharyngeal carcinoma; 35 tissue biopsies from nasal inflammatory polyps and 30 nasopharyngeal

tissues with unremarkable pathological changes, as apparently healthy tissuecontrol. Detection of HHV-8

was done by chromogenic in situ hybridization (CISH) technique detection system.

Results: In nasopharyngeal carcinoma tissues, the HHV-8- DNA positive CISH reactions were detected in

23.1% while in nasal inflammatory polyps tissues HHV-8-positive CISH reactions were found in 8.6% of

the examined tissues. The correlation between HHV-8 and NPC & INP was highly significant (P= 0.001).

Conclusion: Significant HHV-8 detection in nasopharyngeal carcinoma & inflammatory nasal polypstissues

could point for their possible role in either pathogenesis or carcinogenesis of both these lesions.

Author Biography

Zainab A. Hamid1 , Zahraa Ali Abdullah2 , Isra’a Mahdi Al-Sudani3 , Saad Hasan Mohammed Ali4, Shakir H. Mohammed Al-Alwany5 , Khalil Ismail A. Mohammed

1Assist. Prof., College of Medicine/University of Baghdad/Iraq, 2Assist. Lec., Assist. Prof., College of Science/

University of Babylon/Iraq, 4 Prof., College of Medicine/University of Baghdad/Iraq, 5 Prof., College of Science/

University of Babylon/Iraq, 3College of Medicine/University of Ibn Sinafor Medical & Pharmaceutical Science/

Published

2020-07-30

How to Cite

Zainab A. Hamid1 , Zahraa Ali Abdullah2 , Isra’a Mahdi Al-Sudani3 , Saad Hasan Mohammed Ali4, Shakir H. Mohammed Al-Alwany5 , Khalil Ismail A. Mohammed. (2020). In situ Molecular Hybridization of Kaposi, s Sarcoma Associated Virus (Human Herpes Virus 8) in Nasopharyngeal Carcinoma Tissues. Indian Journal of Forensic Medicine & Toxicology, 14(3), 2512-2518. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10814