Preparation of Nano-Antigen for Brucella Abortus by Ultrasound Technique and Evaluate their Sensitivity by ELISA Technique

Authors

  • Dania Dhafer Hameed1 , Siham Sabah Abdullah2 , Ali Abdullhussein Mahdi3

Keywords:

Nanotechnology, Brucellosis, ELIZA Technology

Abstract

Background: The nanoparticle technology, or nanotechnology is the science that studies the treatment of

matter on the atomic and molecular scale. Nanotechnology deals with measurements between 1 to 100

nanometers, i.e. deals with atomic clusters ranging from five atoms to a thousand atoms. They are much

smaller than bacteria and a living cell palsy.

Method: In this study, it involved the production of a new technique for diagnosing infection with Brucella

bacteria. This study included 45 samples of patients suffering from brucellosis (women and men), in addition

to 36 samples from other Brucella. All were diagnosed Injuries by hospital medical advisory staff using

various tests vitek, ELISA, API20C, Morphology, Brucella media test. The samples were examined using

the new technique compared to the routine method of diagnosis. We found that the new technique gave a

positive result to the samples infected with Brucella bacteria, while the rest of the samples without infection

with Brucella bacteria gave a negative result.

Results: It was found that the tests of Elisa and API20C, Vitek were more sensitive 100% and specialized

100% for the diagnosis of Brucella bacteria than other method morpholgy with allergic 44%and specialized

86%, Brucella mediaTest, with allergy 88 % And specialty 86%. The results of the ELISA kit test were the

nano-labs (the new technique) in this study to diagnose more successful bacteria compared to normal system

and more allergic and specialized because of the antigen-containing.

Conclusion: The new kit use in nanotechnology is more Sensitivity and Specificity for diagnosis to the

brucella, Roads routine to diagnose brucella is not effective compared with API, Vitek and ELISA .

Author Biography

Dania Dhafer Hameed1 , Siham Sabah Abdullah2 , Ali Abdullhussein Mahdi3

1 Post Graduate, 2Assist. Prof., Department of Physiology and Medical Physics, college of Medicine,Al-Nahrain

University, Baghdad, Iraq, 3 Assist. Prof., Department of Pathological Analyzes, College of Health and Medical

Technologies University, Baghdad, Iraq

Published

2020-07-30

How to Cite

Dania Dhafer Hameed1 , Siham Sabah Abdullah2 , Ali Abdullhussein Mahdi3. (2020). Preparation of Nano-Antigen for Brucella Abortus by Ultrasound Technique and Evaluate their Sensitivity by ELISA Technique. Indian Journal of Forensic Medicine & Toxicology, 14(3), 2519-2524. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10816