Relation of Endothelin-1 and Malondialdehyde with Pre-eclampsia in Pregnant Women

Authors

  • Rhama Hamid Kassar1 , Baedaa Abdulaziz Salman2 , Fatehiya Majeed Noori2

Keywords:

Endothelin-1; MDA; pre-eclampsia; Pregnancy

Abstract

A case-control study was conducted in Kirkuk city in Kirkuk general hospital from first of March 2018 and

to end of September 2018 to evaluate the role of endothelin-1 and malondialdehyde in pathogenesis of pre

eclampsia in pregnant women. The study included 30 pregnant women with preeclampsia, patients were

defined and divided according to the National High Blood Pressure Education Program Working Group on

High Blood Pressure in Pregnancy. A control group of 30 healthy normotensive pregnant females was also

included. All comparison groups were matched for mother’s age, parity, and gestational age at the time of

enrollment and blood collection. Three ml of blood was collected by vein puncture, blood samples were

placed into sterile test tubes and left for 30 minutes at 37 °C then were centrifuged at 3000 rpm for 15

minutes then the clot was removed and the obtained sera were then aspirated using automatic micropipette

and transferred into clean test tubes and stored in deep freeze at -20°C for determination of endothelin-1 and

malondialdehyde by usingELISA technique. The study showed that the highest mean level of endothelin-1

was found in pregnant women with pre-eclampsia(13.15±2.81 pg/ml) and the lowest mean was in the

control group (0.41±0.31 pg/ml). The result was highly significant (P<0.01). The study showed that the

highest mean level of MDA was found in pregnant women with pre-eclampsia (6.75±2.94 nmol/ml) and

the lowest mean was recorded in the control group (3.85±1.29 nmol/ml). The result was highly significant

(P<0.01). The current study showed that the mean age of patients enrolled in the study was 33.5 years, the

highest mean of endothelin-1 in HD patients was recorded within the age group 20-29 year of pregnant

women with pre-eclampsia. Although the results was non-significant. The study showed positive correlation

between endothelin-1 and MDA levels pregnant women with pre-eclampsia. It was concluded that serum

endothelin-1 and MDA levels were highly correlated with pregnant women with pre-eclampsia

Author Biography

Rhama Hamid Kassar1 , Baedaa Abdulaziz Salman2 , Fatehiya Majeed Noori2

1 M. Sc. Medical Biochemistry, Kirkuk Health Directorate, Kirkuk, Iraq,

2M.B.Ch.B, HD in Obstetric and Gynecology Kirkuk Health Directorate, Kirkuk, Iraq

Published

2020-07-30

How to Cite

Rhama Hamid Kassar1 , Baedaa Abdulaziz Salman2 , Fatehiya Majeed Noori2. (2020). Relation of Endothelin-1 and Malondialdehyde with Pre-eclampsia in Pregnant Women. Indian Journal of Forensic Medicine & Toxicology, 14(3), 2639-2643. Retrieved from http://medicopublication.com/index.php/ijfmt/article/view/10836