Molecular Detection of Antibiotics Resistance Genes in Burkholderia cepecia isolated from Diabetic foot infection

Authors

  • Maryam Riyadh Al-Muhanna1 , Mahdi Al-Ammar2

DOI:

https://doi.org/10.37506/ijfmt.v14i4.12025

Keywords:

Bcc, Burkholderia cepacia ; Antibiotics Resistance; Dabetic

Abstract

This study aimed to isolate and diagnose Burkholderia cepetia from clinical specimens of diabetic foot and
study the resistance of bacteria to antimicrobial agent in Najaf governorate from Augest 2019 to November
2019, which includes 120 clinical specimens for both sexes with an age ranged between )35-70) years
old. The diagnosis of bacteria isolates was based on microscopy, as well as the culture and biochemical
characteristics as an initial diagnosis. The final diagnosis by the Vitek-2 compact system .
Burkholderia cepacia 8(6.6%). Antibiotic sensitivity test was examined by dick diffution method,
Burkholderia cepacia isolates showed high level of resistance almost for all ?-lactam antibiotic classes
under study which included;, ceftriaxone, cefoxitin and Cefepime, with percentage of (100%); ticarcllin
with clavulalnic acide ,piperacllin , ceftazidime, tobramycine, ciproflaxcin and levofloxacin with percentage
of (87.5%); aztreonam and amikacine with percentage(62.5); meropenem , imipenem and gentiamycin with
percentage of (37.5%).
At molecular study, the investigated the presence of antibiotic-resistant genes (blaImp, blaOxa,
blaKpc,blaCTX-M) using PCR technique and electrophoresis systems. 6/8 Burkholderia cepacia isolates
were with blaOXA and (6/8) of isolates carry blaCTX-M gene, all isolates of Burkholderia cepacia gave
negative result of blaIMP and blaKPC gene. Finally PCR analysis showed that the integron gene was (3/8
%).

Author Biography

  • Maryam Riyadh Al-Muhanna1 , Mahdi Al-Ammar2

    1Post graduate, 2Prof. University of Kufa-Faculty of Science-Department of Biology/Iraq

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Published

2020-10-29

How to Cite

Molecular Detection of Antibiotics Resistance Genes in Burkholderia cepecia isolated from Diabetic foot infection . (2020). Indian Journal of Forensic Medicine & Toxicology, 14(4), 2869-2873. https://doi.org/10.37506/ijfmt.v14i4.12025