The Effect of Mixed Liquor Administration on The Johnsen’s Score and The Number of Sertoli Cells and Leydig Cells on The Wistar Strain White Rats (Rattus norvegicus)

Authors

  • Aqidah Khariri1 , Anny Setijo Rahaju2,4, Arifa Mustika3

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13521

Keywords:

mixed liquor, spermatogenic cells, Sertoli cells, Leydig cells.

Abstract

To analyze the effect of mixed liquor administration on the Johnsen’s score and the number of Sertoli cells
and Leydig cells on the Wistar strain white rats (Rattus norvegicus). A total of 28 rats were divided into 4
groups: C, P1, P2, and P3. Rats were given mixed liquor with different dose, namely P1 (1 ml), P2 (2 ml),
and P3 (4 ml) each day for 14 days, then compared to C group which was given 4 ml of distilled water using
an oral gavage. Mixed liquor consists of 20% ethanol and 4% methanol. The histopathological features were
evaluated by the Johnsen’s score, the number of Sertoli cells, and Leydig cells in cross-sectional preparation
of rat testicular tissue with 400× magnification. Datas were analyzed using the Kruskal-Wallis test and OneWay ANOVA test with a confidence level of p <0.05. The P3 group had the lowest Johnsen’s score and the
number of Sertoli cells, 6.442±0.293 and 5.942±0.674, respectively. A significant decrease in increased dose
occurred in the Sertoli cell count but not in the Johnsen’s score. Group P2 had the lowest number of Leydig
cells, 6.421±0.360. The administration of mixed liquor caused a decrease in Johnsen’s score and the number
of Sertoli cells and Leydig cells on the Wistar strain white rat (Rattus norvegicus).

Author Biography

Aqidah Khariri1 , Anny Setijo Rahaju2,4, Arifa Mustika3

1
Student, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, 2Lecturer, Department of Anatomical
Pathology, Faculty of Medicine, Universitas Airlangga Surabaya, Indonesia, 3Lecturer, Department of
Pharmacology and Therapy, Faculty of Medicine, Universitas Airlangga Surabaya, Indonesia, 4Lecturer, Dr.
Soetomo General Academic Hospital, Surabaya, Indonesia

Published

2020-12-31

How to Cite

Aqidah Khariri1 , Anny Setijo Rahaju2,4, Arifa Mustika3. (2020). The Effect of Mixed Liquor Administration on The Johnsen’s Score and The Number of Sertoli Cells and Leydig Cells on The Wistar Strain White Rats (Rattus norvegicus). Indian Journal of Forensic Medicine & Toxicology, 15(1), 840-846. https://doi.org/10.37506/ijfmt.v15i1.13521