Study the effect of purified alginate from Pseudomonas aeruginosa on Hep G2 and A549 cell lines.

Abstract

Alginate was extracted from highest alginate producer of P. aeruginosa 29 isolate, it was determined by
biochemical method (Carbazole-borate assay) and molecular method (PCR), where the amplified algD gene
samples showed that clear bands at one level for all the produced isolates and molecular weight were 313 on
the leader scale. partially purified of alginate using gel filtration chromatography (Sephadex G-200) showed
the present of single peak and initial alginate fraction at 17 to last fraction 28 of alginate concentration ,
where the highest alginate concentration was at fraction 23 . The cytotoxic effect of different concentrations
of purified alginate on cancer cells (HePG2 , A549 ) and normal cells WRL68 was tested. The result of
half-maximal inhibition concentration (IC 50) values of purified alginate treated WRL68 and HepG2 cells
after 24,48,72 hours of incubation at 37º C were ( 187.8 , 62.0, 83.07) ?g /ml , (102.3, 42.5, 61.03) ?g/ml
as respectively. While, the IC50 of purified alginate treated WRL68 and A549 cells after 24,48,72 hours of
incubation at 37ºC were ( 126.2, 63.57, 90.31 ) ?g /ml , (117.8, 57.06, 65.7) ?g /ml as respectively.