Effect of Malus sylvestris Extract on Histopathological Features of Hypercholesterolemic Wistar Rat (Rattus norvegicus) Fatty Liver
Keywords:Fatty Liver, Histopathological, Hypercholesterolemic, Malus sylvestris, Rat.
Objective: To evaluate the effect of Malus sylvestris extract on improving the degree of steatosis and portal
inflammation histopathological features of hypercholesterolemic Rattus norvegicus strain wistar. Method:
Forty-two male rats were divided into 6 groups randomly. Hypercholesterolemic fatty liver induced by
giving high-fat diet (HFD) for 46 days on groups 1 to 5, while group 6 given standard diet with the same
amount of time. Simvastatin was administered in group 2 at a dose of 0.36 mg/day. Groups 3, 4, and 5 were
given Malus sylvestris extract (MSE) as a treatment at doses of 90 mg/day, 180 mg/day, and 360 mg/day in
sequential order. Simvastatin and MSE were administered for 14 days, from day 33 to 46. On day 47, all rats
were sacrificed and the liver was removed for histopathological slides preparation with hematoxylin-eosin
(HE) staining. Histopathological results were analyzed using Kruskal Wallis test followed by Mann Whitney
test. Result: Histopathological analysis showed that Malus sylvestris extract improved steatosis and portal
inflammation features compared to HFD-fed rats in group 1 (p<0,05). Conclusion: Malus sylvestris extract
improved the degree of steatosis and portal inflammation histopathological features of hypercholesterolemic
rat fatty liver.
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