Molecular& Antifungal Suceptibility Identification of Candida albicans Isolated from Samples of GIT Children with Diarrhea in Diyala Province Iraq
Keywords:C.albicans, Nested PCR, Antifungal suceptibility genotype identification
Diarrheal diseases in children are a major public health concern in developing countries. especially species
of Candida, responsible for causing candidiasis infection(diarrehea), require fast and accurate identification,
The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical
significance, allowing an early and appropriate antifungical therapy. This study was conducted to investigate
the incidence of Candida albicans associated with diarrhea in children
This study was included isolates of Candida spp. from stool of 100 patients samples with diarrhea and
100 without diarrhea evaluated in AL-Batool Teaching Hospital by phenotypic methods , antifungal
susceptibility of C. albicans and determination of the C.albicans genotypes by Nested PCR, discriminating
C. albicans from the other Candida species. The tests used for phenotypic analysis were culture in SDA and
CHROMAgar™ Candida, Phenotypic tests showed green colonies in chromogenic medium, The antifungal
susceptibility results of 43 C.albicans isolates present that all of Candida isolates tested were susceptible
to Amphotericin B and fluconazole (100%), while no resistance was observed in C.albicans toCaspofungin
Finally, only one isolate was resistant to flucytosine . Genomic DNAs of all C. albicans isolates were
amplified by PCR to detect their genotypes using. polymerase chian reaction amplification shows four
genotypes(A,B,Cand D).A-genotype showed 4 subtypes and most common subgenotypes(1,3,4 and 5).
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