Phylogenetic Analysis of TTV (TorqueTeno Virus) in Iraqi Woman Suffering of Failure Kidney Disease

Authors

  • YasameenHasan Ali1 , Tariq M Quasim2 , DBEL Wahab R.Alshaikly3

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13648

Keywords:

kidney failure disease,Torque Teno virus (TTV), PCR- Sequencing.

Abstract

There are still very few studies of the Torque Tenovirus infection rate between hemodialysis patients in
Iraq. Thus, the aim of this study was to assess the frequency of TTV viremia in hemodialysis Iraqi patients.
This study has carried out in Baghdad city from October 2019 to February 2020 and included 150 patients
with kidney failure disease undergoing hemodialysis and 50 control subjects .Blood samples have been
obtained for serological TTV detection. The result shows PCR has been used to detect TTV, isolates have
been identified through N22 region sequencing and phylogenetic analysis has also been performed. Of the
150 patients, 2 (1 male and 1 female) were TTV positive.The results shows in ( isolate 14) substitution
four Transversion A>C, C>G,A>T, and T>A three Transition T>C,A>G, and T>C of Specific region within
TTV(N-22) and showed 94% identified with a standard in Gene Bank while having 99% identified with
a standard in Gene Bank with (isolate 17) substitution fiveTransversion A>T,A>C,T>A, A>Tand C>G
threeTransition A>G,A>G and T>C . The phylogenetic analysis showed that identical among themselves
and the world with 100% compatibility values.

Author Biography

YasameenHasan Ali1 , Tariq M Quasim2 , DBEL Wahab R.Alshaikly3

1
Assistant Professor College of Nursing, 2Tutor Department of pharmacy, Al-Rasheed University College
3
Prof

Published

2020-12-31

How to Cite

YasameenHasan Ali1 , Tariq M Quasim2 , DBEL Wahab R.Alshaikly3. (2020). Phylogenetic Analysis of TTV (TorqueTeno Virus) in Iraqi Woman Suffering of Failure Kidney Disease. Indian Journal of Forensic Medicine & Toxicology, 15(1), 1659-1664. https://doi.org/10.37506/ijfmt.v15i1.13648