Phylogenetic Analysis of TTV (TorqueTeno Virus) in Iraqi Woman Suffering of Failure Kidney Disease
DOI:
https://doi.org/10.37506/ijfmt.v15i1.13648Keywords:
kidney failure disease,Torque Teno virus (TTV), PCR- Sequencing.Abstract
There are still very few studies of the Torque Tenovirus infection rate between hemodialysis patients in
Iraq. Thus, the aim of this study was to assess the frequency of TTV viremia in hemodialysis Iraqi patients.
This study has carried out in Baghdad city from October 2019 to February 2020 and included 150 patients
with kidney failure disease undergoing hemodialysis and 50 control subjects .Blood samples have been
obtained for serological TTV detection. The result shows PCR has been used to detect TTV, isolates have
been identified through N22 region sequencing and phylogenetic analysis has also been performed. Of the
150 patients, 2 (1 male and 1 female) were TTV positive.The results shows in ( isolate 14) substitution
four Transversion A>C, C>G,A>T, and T>A three Transition T>C,A>G, and T>C of Specific region within
TTV(N-22) and showed 94% identified with a standard in Gene Bank while having 99% identified with
a standard in Gene Bank with (isolate 17) substitution fiveTransversion A>T,A>C,T>A, A>Tand C>G
threeTransition A>G,A>G and T>C . The phylogenetic analysis showed that identical among themselves
and the world with 100% compatibility values.
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