Cytotoxicity of lipopolysaccharide extracted from Salmonella enterica serovar Typhimurium on Breast Cancer Cell Line mcf-7
Keywords:Lipopolysaccharide, S. enterica serovar Typhimurium, MCF-7cell line, cytotoxicity.
Lipopolysaccharide was selected to investigate its effect on cell line of breast cancer in vitro, therefore
lipopolysaccharide was extract from Salmonella enterica serovar Typhimurium followed by evaluation of its
cytotoxicity. The experiment was divided into three parts. The first part included isolation and identification
of S. enterica serovar Typhimurium from 302 samples included 201diarrheic patient samples and 101 food
samples of poultry meat and as well as the isolates identification on molecular level through used of three
genes (invA, STM4497 and Stn) genes detected using the technique of PCR, the results shown there were
seven bacterial isolates diagnosed as S. enterica serovar Typhimurium. The second part of the experiment
was extract of LPS from 10.66 g of dry weight of bacteria by hot phenol method. The concentration of LPS
in phenolic phase was 435.57?g/ml while in aqueous phase was 381.76 ?g/ml. The LPS molecular weight
was estimate which was equivalent to 93325 for phenolic and 71614 Dalton for aqueous phases. The third
part of experiment was to study the effect of cytotoxicity in cell line of breast cancer by used different
concentrations of lipopolysaccharide. Lipopolysaccharide showed cytotoxicity on the breast cancer cell line
mcf-7 in both phenolic and aqueous phases of LPS. The results showed the highest rate of inhibition of
breast cancer cells at the concentration of 350 ?g/ml by 61.67% in the phenolic phase while the rate of
inhibition was 53.33% in the same concentration of the aqueous phase.
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