Detection of HMA5, PCs and MT2 Genes Expression in Vicia faba Under Heavy Metal Stress Using Quantitative Real-Time PCR

Authors

  • Khalid H. Alobaidi1 , Zaid N. Altememi2 , Dhafar N. Al-Ugaili3 , Jameel R. Al-Obaidi4

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13716

Keywords:

Vicia faba, HMA5, PCs, MT2, copper, zinc, RT-qPCR.

Abstract

In order to achieve phytoremediation during agricultural production, it is essential to identify high genotypes
yield that is able of accumulating many types of heavy metals not in the edible parts which have significant
nutritional value but in the non-edible parts. This study conducted to estimate the heavy metal ATPases 5
(HMA5), Phytochelatins synthase (PCs) and metallothionein 2 (MT2) genes expression in plant Vicia faba
in response to an elevated concentration of copper and zinc in nutrient media. Using Quantitative RealTime PCR (RT-qPCR) technique, the results of hydroponic culture methods with high concentrations of
copper (75 µMol / L) and high concentration of zinc (500 µ Mol / L) showed high expression level for the
three genes of Vicia faba plant compared with control. Established that the expression of the genes under
the influence of copper ion was higher than the expression under zinc ion influence. Besides that, gene
expression increased with increased exposure time to zinc ion, also in the case of copper ion exposure time,
all genes expression slightly increases with increased exposure time. In response to excess copper and zinc,
an increase in the expression of genes (HMA5, PCs and MT2) involved in plant protection, providing the
possibility of its transfer from the cytosol to the apoplast demonstrate that this plant might be useful for
phytoremediation of moderately polluted areas with copper or zinc.

Author Biography

Khalid H. Alobaidi1 , Zaid N. Altememi2 , Dhafar N. Al-Ugaili3 , Jameel R. Al-Obaidi4

1
Assistance Professor,Department of Plant Biotechnology, College of Biotechnology, Al-Nahrain University, Iraq,
2Lecturer, 3
Assistance Lecturer, Department of Molecular and Medical Biotechnology, College of Biotechnology,
Al-Nahrain University, Iraq, 4Senior Lecturer, Faculty of Science and Mathematics, Universiti Pendidikan Sultan
Idris, 35900 Tanjong Malim, Perak. Malaysia

Published

2020-12-31

How to Cite

Khalid H. Alobaidi1 , Zaid N. Altememi2 , Dhafar N. Al-Ugaili3 , Jameel R. Al-Obaidi4. (2020). Detection of HMA5, PCs and MT2 Genes Expression in Vicia faba Under Heavy Metal Stress Using Quantitative Real-Time PCR. Indian Journal of Forensic Medicine & Toxicology, 15(1), 2102-2108. https://doi.org/10.37506/ijfmt.v15i1.13716