Detection of Tn916 Conferring Tetracycline Resistance in Clinical Isolates of Streptococcus pyogenes
Keywords:Tn916, S.pyogenes, Transposable elements, Antibiotic resistance .
Background: During the past twenty years, tetracycline resistance average has increased in Streptococcus
pyogenes in many countries. Pneumococcal resistance to erythromycin and tetracycline is associated with
the insertion of the erm(B) into the transposons of the Tn916 family. To get datum that may be beneficial in
resolving the diffusion of antimicrobial resistance, therefore, we can be specified antibiotic resistant genes
and their corporation with mobile genetic elements. This study was proceed to explore the genetic regulation
of Tn916-carrying tet(M) in clinical isolates of S.pyogenes . Methods: A twenty two of S.pyogenes isolates
were assemble from patients suffering from upper respiratory infection, and the susceptibility of these
isolates to tetracycline antibiotics was examined. Molecular detection of Tn916 was carried out by employ
certain primers to amplify tet(M) gene in each isolates.
Results: The results appeared that the resistance of the tetracycline group was 68.1%, 54.5%, 36.3% and
31.8% for minocycline, tetracycline, oxytetracycline and doxycycline, respectively. Genetic analysis showed
that Tn916 was detected in eight of the S.pyogenes clinical isolates resistant to tetracycline. Conclusions:
Our findings suggest that clinical isolates of S. pyogenes harboring a copy(s) of Tn916 conferring tetracycline
resistance. One possible explanation for resistance to tetracycline in these isolates is due to tet gene, which
was most likely located on Tn916.
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