Novel Molecular Detection of BPV-10 and BPV-11 Genotypes in Al- Anbar, and Baghdad Provinces
Keywords:BPV-10, BPV-11, Genotypes, Molecular detection, PCR
In this study, the prevalence of diverse bovine papilloma viruses in cutaneous lesions was investigated in Iraqi
cows. Fourteen specimens were taken from udder growths of Iraqi dairy cows, which demonstrated rice grain-like
and small, raised flesh-like appearance. DNA was extracted from the teat wart samples, then was amplified by the
polymerase chain reaction (PCR) using primers against BPV-10 (403 bp) and BPV-11 (560 bp). PCR results revealed
mixed infections of BPV-10 and BPV -11 in four samples, while the remaining ten samples were infected with BPV10 only. These findings demonstrate the presence of bovine papillomaviruses 10 and 11 in rice grain-like cutaneous
lesions on the teats of cows and multiple elevated growths on udder. The detection and report of the BPV-10 and
BPV -11 genotypes in the current study is for first time in Iraq, which are known in the Middle East region, are crucial
for disease control measurements.
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