Novel Molecular Detection of BPV-10 and BPV-11 Genotypes in Al- Anbar, and Baghdad Provinces

Authors

  • Mohammed A. Hamad1 , Murtadha A. Al-Mudhafr2 , Ahmed Majeed Al-Shammari3, Ahmed J. Neamah4

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13722

Keywords:

BPV-10, BPV-11, Genotypes, Molecular detection, PCR

Abstract

In this study, the prevalence of diverse bovine papilloma viruses in cutaneous lesions was investigated in Iraqi
cows. Fourteen specimens were taken from udder growths of Iraqi dairy cows, which demonstrated rice grain-like
and small, raised flesh-like appearance. DNA was extracted from the teat wart samples, then was amplified by the
polymerase chain reaction (PCR) using primers against BPV-10 (403 bp) and BPV-11 (560 bp). PCR results revealed
mixed infections of BPV-10 and BPV -11 in four samples, while the remaining ten samples were infected with BPV10 only. These findings demonstrate the presence of bovine papillomaviruses 10 and 11 in rice grain-like cutaneous
lesions on the teats of cows and multiple elevated growths on udder. The detection and report of the BPV-10 and
BPV -11 genotypes in the current study is for first time in Iraq, which are known in the Middle East region, are crucial
for disease control measurements.

Author Biography

Mohammed A. Hamad1 , Murtadha A. Al-Mudhafr2 , Ahmed Majeed Al-Shammari3, Ahmed J. Neamah4

1
Assistant Professor, Biotechnology and Environmental Center, University of Fallujah, Al-Anbar 31002, Iraq,
2
Assistant Professor, University of Kufa, Faculty of Veterinary Medicine, Department of Microbiology, 3
Assistant
Professor, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad 1001, Iraq,
4 Professor, College of Veterinary Medicine, Al Qadisiyah University

Published

2020-12-31

How to Cite

Mohammed A. Hamad1 , Murtadha A. Al-Mudhafr2 , Ahmed Majeed Al-Shammari3, Ahmed J. Neamah4. (2020). Novel Molecular Detection of BPV-10 and BPV-11 Genotypes in Al- Anbar, and Baghdad Provinces. Indian Journal of Forensic Medicine & Toxicology, 15(1), 2141-2147. https://doi.org/10.37506/ijfmt.v15i1.13722