Stimulation of Staphylococcus aureus Ligase Enzyme by Magnesium Ion

Authors

  • Arqam Mohamad Alomari1 , Aseel A. H. Al-Layla2 , Ibrahim Faris Ali3

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13723

Keywords:

DNA ligases, Magnesium ion, Staphylococcus aureus, Michaelis-Menten equation.

Abstract

Ligases enzymes were discovered as a member of the nucleotidyl transferase family. Here in this paper, DNA
Ligase is extracted from S. aureus works with the cofactor NAD+ to make a phosphodiester bond and reform
between the 3’hydroxyl and 5’phosphate DNA end. Staphylococcus aureus-DNA Ligases Enzyme type A
(SLE-A) contains two essential domains; NTase and OB- fold domain, which are the most essential domains
for the enzyme function. The main aim of the study is to investigate the activity of SLE-A in the presence
of magnesium ion (Mg+2) by evaluating several kinetic parameters on a time course. The result showed that
SLE-A has optimal activity at 500 µM of Mg+2. Furthermore, the low number of Equilibrium Association
Constant (Km value) explains the binding affinity between DNA ligase of Staphylococcus aureus SLE-A
enzyme and Mg+2 ion was very high and sold.

Author Biography

Arqam Mohamad Alomari1 , Aseel A. H. Al-Layla2 , Ibrahim Faris Ali3

1
Lecturer, Department of Basic Sciences, College of Agriculture and Forestry, University of Mosul, Mosul, Iraq,
2
Department of Biophysics, College of Sciences, University of Mosul, Mosul, Iraq, 3
Department of Biology, College
of Education for Pure Science, University of Mosul, Mosul, Iraq

Published

2020-12-31

How to Cite

Arqam Mohamad Alomari1 , Aseel A. H. Al-Layla2 , Ibrahim Faris Ali3. (2020). Stimulation of Staphylococcus aureus Ligase Enzyme by Magnesium Ion. Indian Journal of Forensic Medicine & Toxicology, 15(1), 2148-2156. https://doi.org/10.37506/ijfmt.v15i1.13723

Issue

Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology

Section

Articles
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