The Effect of Dual-Species Biofilms, Monosaccharide and D-Amino Acids on Pseudomonal Biofilm

Authors

  • Al-Moghira Khairi Al-Qaysi1 ; Safaa Abed Latef Al-Meani2 ; Mushtak T.S. Al-Ouqaili3

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13728

Keywords:

Biofilm, monosaccharide, D-amino acids

Abstract

Background: The major therapeutic failure in clinical settings is due to problematic biofilm-producing
bacteria like Pseudomonas aeruginosa. This study aims to investigate the effect of dual-species biofilms,
monosaccharide, and D-amino acids on pseudomonal biofilm. Methodology: A total of 130 patients with
catheter-associated urinary tract and wound infections were involved in this study. Quantitative biofilm
assay by alginate beads was performed. The dual-species biofilms have been done, and the effect of
ciprofloxacin, monosaccharides, and D-amino acids on sessile cells was detected. Conclusion: This study
suggested that the combined action of both monosaccharides (glucose and galactose) and the combination of
each one with ciprofloxacin is the enhancing of P. aeruginosa biofilm and increases survival strategy against
ciprofloxacin. In contrast, a noticeable biofilm reduction and marked synergistic action for ciprofloxacin
have been determined in the combination of the two D-amino acid, alanine, and glycine in comparison to
the effect of each one alone. Furthermore, the dual biofilm of P. aeruginosa with each of K. pneumonia and
E. coli, markedly reduced pseudomonal biofilm, while co-culture with S. aureus revealed strong support of
pseudomonal pathogenicity and increased its biofilm production.

Author Biography

Al-Moghira Khairi Al-Qaysi1 ; Safaa Abed Latef Al-Meani2 ; Mushtak T.S. Al-Ouqaili3

1
Assistant lecture, University of Anbar, College of Education for Pure Sciences, Department of Biology,
2
Assistant Professor, University of Anbar, College of Science, Department of Biotechnology, 3
Professor, University
of Anbar, College of Medicine, Department of Microbiology

Published

2020-12-31

How to Cite

Al-Moghira Khairi Al-Qaysi1 ; Safaa Abed Latef Al-Meani2 ; Mushtak T.S. Al-Ouqaili3. (2020). The Effect of Dual-Species Biofilms, Monosaccharide and D-Amino Acids on Pseudomonal Biofilm. Indian Journal of Forensic Medicine & Toxicology, 15(1), 2177-2192. https://doi.org/10.37506/ijfmt.v15i1.13728