The Effect of Dual-Species Biofilms, Monosaccharide and D-Amino Acids on Pseudomonal Biofilm
Keywords:Biofilm, monosaccharide, D-amino acids
Background: The major therapeutic failure in clinical settings is due to problematic biofilm-producing
bacteria like Pseudomonas aeruginosa. This study aims to investigate the effect of dual-species biofilms,
monosaccharide, and D-amino acids on pseudomonal biofilm. Methodology: A total of 130 patients with
catheter-associated urinary tract and wound infections were involved in this study. Quantitative biofilm
assay by alginate beads was performed. The dual-species biofilms have been done, and the effect of
ciprofloxacin, monosaccharides, and D-amino acids on sessile cells was detected. Conclusion: This study
suggested that the combined action of both monosaccharides (glucose and galactose) and the combination of
each one with ciprofloxacin is the enhancing of P. aeruginosa biofilm and increases survival strategy against
ciprofloxacin. In contrast, a noticeable biofilm reduction and marked synergistic action for ciprofloxacin
have been determined in the combination of the two D-amino acid, alanine, and glycine in comparison to
the effect of each one alone. Furthermore, the dual biofilm of P. aeruginosa with each of K. pneumonia and
E. coli, markedly reduced pseudomonal biofilm, while co-culture with S. aureus revealed strong support of
pseudomonal pathogenicity and increased its biofilm production.
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