Detection of Fungal Toxins Produced by Dermatophytes by using Thin Layer Chromatography

Authors

  • Abeer Mohammed Ali Al-garawyi1 , Adnan H. Al-Hamadani2 , Adnan waheed AL- Bederi3

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13766

Keywords:

E. floccosum , Dermatophytosis, TLC , Mycotoxins

Abstract

Dermatophyte fungi include a wide range of filamentous fungi that are pathogenic to humans including
three superficial cutaneous genera such as ; Epidermophyton, Microsporum and Trichophyton. However,
the aim of this study isolation and identification of fungi responsible of dermatophytosis and detection of
the dermatophyte ability to produce mycotoxins. The results of this study showed that 49(69.01%) out of
71 specimens were gave positive results , while 22 (30.98%) were gave negative results by examination
on 10%KOH and cultured on Sabouraud dextrose agar with cyclohexmaide. Out of 49 culture positive
isolates, 31(63.3%) T. rubrum isolates was the most frequent etiological agent followed by 11( 22.4%)
T.mentagrophytes ; 5(14.3%) M. canis and 2
(4.1%) Epidermophyton floccosum isolates. The use of the TLC method to detect of mycotoxins in chloroform
extracts for 49 dermatophyte mycelia. It was found all dermatophyte isolates are able to produce fluorescent
stains with different colors under UV light at 365 nm , also these stains may be act as a non-enzymatic
virulence indicator ( mycotoxins).

Author Biography

Abeer Mohammed Ali Al-garawyi1 , Adnan H. Al-Hamadani2 , Adnan waheed AL- Bederi3

1
Post graduate Biology Department, Education for Pure Science College, Al-Muthanna University, Iraq, 2 Prof.
Department Microbiology/ College of Medicine / Al-Qadisiyah University, 3
Prof. Department Anatomy/ College of
Medicine / Al-Qadisiyah University

Published

2020-12-31

How to Cite

Abeer Mohammed Ali Al-garawyi1 , Adnan H. Al-Hamadani2 , Adnan waheed AL- Bederi3. (2020). Detection of Fungal Toxins Produced by Dermatophytes by using Thin Layer Chromatography . Indian Journal of Forensic Medicine & Toxicology, 15(1), 2434-2438. https://doi.org/10.37506/ijfmt.v15i1.13766