Evaluation of antioxidants capacity of non-enzymatic antioxidants and its effect in glucose level in diabetic patients

Authors

  • Rana Fadhil Mousa

DOI:

https://doi.org/10.37506/ijfmt.v15i1.13781

Keywords:

Antioxidant, total antioxidant capacity, non-enzymatic antioxidants, diabetes

Abstract

Background: Oxidative stress might participate in the pathophysiology of diabetes type 2. Systems of nonenzymatic antioxidants are made up of scavenging molecules that are formed endogenously.
The Study Objective: This study’s aims were evaluation of the non-enzymatic antioxidants and their effect
in levels of glucose among diabetic patients.
Methodology: A total of eighty patients with DM type 2 and forty healthy persons (control) were enrolled
within this study. Serum and plasma were obtained from collected blood samples. The Ferric Reducing
Ability of Plasma (FRAP) method was used for detection of the total antioxidant capacity (TAC). Ellman’s
method was used to determine the level of reduced glutathione. The serum CAT activity was detected by the
technique explained by Sinha.
Results: The findings revealed that, among diabetic groups, there was a significant elevation in glycosylated
hemoglobin and glucose levels; HbA1c and FBG levels were significantly higher; MDA, NO, LPI, TAC and
GSH showed significant greater values. The activity of SOD showed significantly greater mean value among
the control group.
Conclusion: The results suggest that antioxidants defense might be decreased in T2DM, as TAC levels
were decreased. The increased levels of MDA, NO, LPI, TAC and GSH were associated with oxidative
stress. Assessment of GSH could help in recognition of the extent of oxidative stress in diabetes as well as
prevention and control of diabetic complications.

Author Biography

Rana Fadhil Mousa

Assistant prof., College of Veterinary Medicine / University of Kerbala

Published

2020-12-31

How to Cite

Rana Fadhil Mousa. (2020). Evaluation of antioxidants capacity of non-enzymatic antioxidants and its effect in glucose level in diabetic patients . Indian Journal of Forensic Medicine & Toxicology, 15(1), 2532-2538. https://doi.org/10.37506/ijfmt.v15i1.13781