Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits

Authors

  • Luki Indriaswati
  • Nurwasis
  • Gatut Suhendro
  • Soetjipto
  • Retno Handajani

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14785

Keywords:

caspase-3, degeneration of lateral rectus muscle, esotropia, rabbit

Abstract

Background: To identify correlation between caspase-3 expression and degeneration of lateral rectus muscle
on experimental esotropia in rabbits by using time dependent analysis. Methods: Twenty four rabbits were
divided into 4 groups, first month sham (S1); second month sham (S2); first month esotropia (E1); and
second month esotropia (E2) model groups. Immunohistochemical and haematoxyllin eosin staining were
performed. Results: In first month observation no significant difference in caspase-3 expression between S1
(1.56 ± 1.03) and E1 (3.93 ± 1.66) groups (p >0.05) and in the degeneration score of lateral rectus muscle
between S1 (1.33 ± 1.36) and E1 (3.83 ± 2.04) groups (p> 0.05). In second month observation no significant
difference in caspase-3 expression between S2 (1.80 ±1.08) and E2 (6.50 ± 3.26) groups (p >0.05); but
there was significant difference in the degeneration score of lateral rectus muscle between S2 (1.16 ± 1.16)
and E2 (4.50 ± 1.04) groups (p <0.05). There was a significant correlation between caspase-3 expression
and the degeneration score of lateral rectus muscle (p=0.020; r=0.471). Conclusion: On the experimental
esotropia with prolonged lateral muscle contraction in rabbits, the increasing trend of caspase-3 expression
has a correlation with the increasing degeneration score of lateral rectus muscle.

Author Biographies

  • Luki Indriaswati

    Doctoral student

  • Nurwasis

    Lecturer

  • Gatut Suhendro

    Professor, Department of Ophthalmology

  • Soetjipto

    Professor

  • Retno Handajani

    Professor, Department of
    Biochemistry, Faculty of Medicine, Universitas Airlangga

Downloads

Published

2021-03-24

How to Cite

Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits. (2021). Indian Journal of Forensic Medicine & Toxicology, 15(2), 2730-2735. https://doi.org/10.37506/ijfmt.v15i2.14785