Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice

Authors

  • Ahmad Rukhani Lutfi
  • Lilik Herawati
  • Widjiati
  • Gadis Meinar Sari

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14944

Keywords:

Calorie restriction, moderate-intensity continuous exercise, free fatty acids, visceral fat, cholesterol

Abstract

Objective: This study aimed to analyze the effect of a combination of calorie restriction and moderateintensity continuous exercise on FFA levels and visceral fat weight in female mice exposed to a high-calorie
diet.
Material and Method: This research was randomized posttest used only control group design with female
mice as the sampl Balb/c mice (Mus musculus), 8 weeks old, 20-30 grams. It was randomly divided into four
groups, namely C (control group), G1 (moderate-intensity continuous exercise group), G2 (calorie restriction
group), and G3 (combination group calorie restriction and moderate-intensity continuous exercise) where
in every group consisted six female mice. Providing a high-calorie diet using ad libitum plus D40 as much
as 3-5% of calories (0.0325 mL/gBB) for 4 weeks. The provision of a high-calorie diet was done using
the sonde technique. Calorie restriction was done 3x/week in 4 weeks by limiting calorie intake to 50% of
standard foods. The moderate-intensity continuous exercise was carried out by swimming for 15 minutes
plus 6% gBB loading, it was done 3 times/week for 4 weeks. The measurement of cholesterol, visceral fat
weight, and FFA level was done at the end of treatments.
Results: The results found that the mean of cholesterol of C C (171.00±47.74 mg/dL), G1 (126.00±15.92 mg/
dL), G2 (131.50±22.37 mg/dL), G3 (121.17±21.23 mg/dL) dan (p=0.032). The mean weight of visceral fat
at C (0.68±0.23 grams), G1 (0.25±0.08 grams), G2 (0.28±0.06 grams), G3 (0.20±0.03 grams) and (p=0.000).
The mean FFA levels were at C (1511.34±6.19 µmol/L), G1 (1419.31±53.61 µmol/L), G2 (1458.20±28.89
µmol/L), G3 (1451.55±43.77 µmol/L) and (p=0.004).
Conclusion: Based on the results of the study, it could be concluded that the provision of calorie restriction
and moderate-intensity continuous exercise significantly reduced cholesterol levels, visceral fat weight, FFA
levels and in female mice exposed to a high-calorie diet

Author Biographies

  • Ahmad Rukhani Lutfi

    Master, Sports Health Science, Faculty of Medicine Airlangga University

  • Lilik Herawati

    Lecturer, Department of Physiology,
    Faculty of Medicine Airlangga University

  • Widjiati

    Professor, Faculty of Veterinary Medicine, Airlangga University

  • Gadis Meinar Sari

    Lecturer, Department of Physiology,
    Faculty of Medicine Airlangga University

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Published

2021-03-24

How to Cite

Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice. (2021). Indian Journal of Forensic Medicine & Toxicology, 15(2), 3665-3677. https://doi.org/10.37506/ijfmt.v15i2.14944