Suitability Tests for Bacterial Identification and Antibiotic Sensitivity Tests using Microscan Walkaway on Vitek 2
DOI:
https://doi.org/10.37506/ijfmt.v15i2.14995Keywords:
Identification of bacteria, antibiotic sensitivity, Microscan WalkAway, Vitek 2Abstract
Culture or bacterial culture is the gold standard for diagnostic examination to detect the presence of
microorganisms in the patient’s body. The development of culture technology and culture-based automatic
devices for diagnostic examination are widely researched. At present, there are several types of automatic
blood culture instruments in Indonesia, namely Vitek 2 and Microscan Walkaway. This research aims to
analyze the suitability of bacterial identification and test antibiotic sensitivity of Microscan Walkaway device
from Beckman Coulter and Vitek 2 device from Biomerieux as a gold standard. The research conducted
was an observational cross-sectional study. Sampling was done consecutively. The study sample consisted
of 202 samples obtained from the results of positive isolates, during January-July 2019 at Dr. Soetomo
hospital. Positive isolates were examined for bacterial identification and antibiotic sensitivity using Vitek
2 and Microscan Walkaway. The results were statistically analyzed using SPSS. Examination of bacterial
identification using the Microscan device showed 34.2% of Gram-positive bacteria and 65.8% of Gramnegative bacteria, whereas with Vitek 2, results showed 34.7% of Gram-positive bacteria and 65.3% of
Gram-negative bacteria. Both of these instruments showed identification accuracy of 98.56% for Gram
positive bacteria and 100% for Gram negative bacteria with Kappa value: 0.814 and p <0.0001. The results
of the accuracy test for antibiotic sensitivity of multidrug resistance bacteria showed compatibility with p
value <0,0001. There is very good agreement between Vitek 2 and Microscan Walkaway in the identification
of bacteria and antibiotic sensitivity.
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