Study of LDH Isoenzymes in Myocardial Infraction at Vidharbha Region (Central India)

Authors

  • Rakesh Kumar Jha
  • Pradip Jain
  • Ankita Khandolkar
  • Archana Dhok

DOI:

https://doi.org/10.37506/ijfmt.v15i2.15084

Keywords:

Lactate Dehydrogenase, Myocardial Infarction, Coronary Artery Disease, Isoenzymes, ECG.

Abstract

Introduction: A significant cause of morbidity and mortality worldwide is myocardial infarction (MI).
Within the first few hours of the beginning of MI, the greatest risk of death occurs. Thus, for the effective
treatment of patients with MI, early detection of cardiac ischemia is essential. Inappropriate diagnosis of
patients with chest pain sometimes leads to improper admission, and vice versa, of patients without MI.
Physical examination, precise ECG results and evaluation of cardiac biomarkers play an important role
in the early diagnosis of acute ischemia, in addition to clinical history. In the present analysis, the cardiac
biomarker lactate dehydrogenase released during a MI event is discussed in depth.
Aim: We conducted a cross sectional study to examine the different levels of LDH isoenzymes in myocardial
infarction patients those admitted in cardiac care unit in SMHRC Nagpur.
Material and Methods: The present study included 100 subjects of age group 30-80 years. Patients admitted
to the coronary care unit at SMHRC & AVBRH between January 2020 and June 2020 was considered
eligible for the study. These guidelines ensured that sufficient blood samples were available and the criteria
for diagnosis were consistent. Lactate dehydrogenase isoenzyme was fractionated by agarose gel electrophoresis as described by Cawley and Eberhard. The stained slides were scanned on Chromoscan at 520 mix
and the percentages of the fractions were quantitated.
Results: The results obtained in the present study indicate that serum lactate dehydrogenase enzyme activity
increases 4-5 folds in the first 24-72 hours after the onset of clinical infarction with a peak on the second day.
It then declines gradually towards the normal within two weeks. Thus, determination of total serum LDH
is valuable if patients present themselves for clinical check up even after a week from the day of infarct.
Although the contribution of myocardium to total serum lactate dehydrogenase is large, its interpretation
becomes difficult if the diseases of other organs such as liver, kidney, etc.
Conclusion: In conclusion, the present data strongly support the use of Lactate dehydrogenase for the
diagnosis of MI. Such a strategy should boost both the precision and sensitivity for myocardial infarction
diagnosis.

Author Biographies

  • Rakesh Kumar Jha

    Tutor, Dept. of Biochemistry Datta Meghe Medical College, Nagpur

  • Pradip Jain

    Professor and HOD, Dept. of Biochemistry
    Datta Meghe Medical College, Nagpur

  • Ankita Khandolkar

    Tutor, Dept. of Biochemistry Datta Meghe Medical College, Nagpur

  • Archana Dhok

    Professor and HOD Dept. of Biochemistry Jawaharlal Nehru Medical
    College, Datta Meghe Institute of Medical Sciences, Sawangi Meghe Wardha

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Published

2021-03-24

How to Cite

Study of LDH Isoenzymes in Myocardial Infraction at Vidharbha Region (Central India). (2021). Indian Journal of Forensic Medicine & Toxicology, 15(2), 4466-4471. https://doi.org/10.37506/ijfmt.v15i2.15084