Extraction and High Purification of Nicotine from Iraqi Tobacco Leaf Formanufacturing, Pharmaceutical, and Medicinal Uses
Keywords:Nicotine , Iraqi tobacco, Lipophilic solvent, TLC, GC-MS chromatography.
Bachground: Nicotine is highly addictive plant derived alkaloid and the most important species in human
use today is Nicotianatabacum. There are direct health effects of chronic nicotine exposure. Even in low
doses, nicotine causes vasoconstriction and other cardiovascular effects related to catecholamine release and
promote angiogenesis, neuroteratogenicity, and possibly some cancers. Methods: A preliminary investigation
to analyze the nicotine contained in Iraqi tobacco leaves was carried out using gas chromatography-mass
spectrometry (GC-MS). Nicotine is an alkaloid, and alkali methanol and Lipophilic solventsystem methods
(LSS) have been extracted and determined by GC-MS from tobacco leaves. Results: The detection limit for
nicotine was for non-selective monitoring at the ppm level and for selective detection at the nanogram level.
This is a simple method of thin layer chromatography (TLC) and chromatography mass spectrometry (GCMS)
for the tobacco leave analysis of nicotine. The final purity of nicotine is 99%. Conclusion :the methods
which used in this study gave very high purity of nicotineafter converting the crude nicotine to its esters.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions