Detection of Mn – Dependent Chitinase for Wheat Root Rot Disease Control by Real time PCR
The use of some mineral salts with concentrations of 10, 30 and 50 mm Muller led to the inhibition of
the activity of the enzyme chitinase, but to varying degrees. Manganese chloride MnCl2 showed more
effect in stimulating the activity of the enzyme, as the effectiveness reached 74.87% in addition to calcium
chloride CaCl2, which had a stimulating effect for the enzyme as well. The salts EDTA and NH4Cl had an
inhibitory effect on the enzyme activity. The field experiment was conducted for the purpose of inducing
systemic resistance of wheat plants with the fungus T. longibrachiatum under conditions of infection with
the pathogen Fusarium oxysporum and evaluating the efficacy of the chitinase enzyme in improving plant
growth for three varieties of wheat, which are Iba 99, Sham 6 and July 2 for 45 days, after which the
vegetative characteristics were taken. For cultivated plants and the efficacy of the chitinase enzyme for the
shoot and root system, the induction treatment (pathogenic fungus + T. longibrachiatum + MnCl2) proved its
role in the highest reduction of the severity of infection with the pathogen fungus of wheat, reaching 18.05%
and the high vegetative and root growth indicators under conditions of pathogen infection and for all the
studied varieties. The results of induction were due to the increased expression of the chitinase enzyme gene.
RT - PCR technique was used. It showed that all the studied cultivars with 99, Sham 6 and July 2 had higher
expression than the control treatment.
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