Study the Cytochrome P450 Gene Expression Changes in Iraqi Patients with Chronic Liver Disease
Keywords:chronic liver disease, cytochrome P450, Fatty acid synthase, gene expression.
The cytochrome P450 is a chemical group of heme-containing proteins speaks to one of the biggest and most practically various superfamilies’ found in nature. Chronic liver disease (CLD) is a the process of the liver that involves a process of progressive destruction and regeneration of the liver parenchyma leading to fibrosis and cirrhosis. The aim of this study to assessment the cytochrome P450 gene expression changes of three variants, CYP1A2, CYP2B6, and CYP2E1 in Iraqi patients with chronic liver disease(hepatitis+ alcoholism and hepatitis+ non-alcoholism).CYP1A2, CYP2B6, and CYP2E1 mRNA gene expression were assessed by quantitative real time PCR (qRT-PCR) in 50 cases with CLD and 50 subjects as control. Primers for genes of interest, CYP1A2, CYP2B6, and CYP2E1mRNA and housekeeping gene (GAPDH) were designed by using NCBI tools. Fatty acid synthase (FAS) was estimated by ELISA technique. The results showing statically significant differences in FAS levels (ng/ml) between study groups, HC, HNC, and control (p-value< 0.05). GEF in CYP1A2 gene showing no change between HC and control but there were statistical variations in GEF in HNC and both HC or control. GEF in CYP2B6 and CYP2E1 genes showing highly significant differences between control and both HNC and HC. In conclusion, CYP2B6 and CYP2E1 gene expression were risk factor for progression HC but not HNC through stimulation of increasing levels of FAS.
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