Phenotypic detection of AmpC ?-lactamase in Pseudomonas aerogenosa isolated from Burns and Wounds in Al-Rumetha Hospitals

Authors

  • Abeer Mohammed Ali Al-garawyi

DOI:

https://doi.org/10.37506/ijfmt.v14i2.2960

Keywords:

Pseudomonas aeruginosa, AmpC, MTDT, Cefoxitin

Abstract

Ampicillin hydrolyzing class C ?-lactamase (AmpC) producing Pseudomonas aeruginosa has been recognized to be a serious opportunistic infection and due to the wide ability to create ?-lactamases made this bacteria resistance to many antibiotics.The results of the current study showed that 36(27.7%) isolates of P. aeruginosa from 130 isolates examined for cefoxitin susceptibility by disk diffusion method. Out of thirty six P. aeruginosa isolates , 29(80.6%) were cefoxitin resistant . The modified three dimension test and AmpC disck test were used confirm the production AmpC ?- lactamase in all cefoxitin resistant isolates , only 23(79.3%) isolates were phenotypically confirmed to produce AmpC beta-lactamase. The result of this study showed that 12/36 ( 33.3%) isolates were positive with blaAmpC gene by conventional PCR technique

Author Biography

Abeer Mohammed Ali Al-garawyi

1Biology Dep./ Education for Pure Science College/Al-Muthanna University

Published

2020-04-29

How to Cite

Abeer Mohammed Ali Al-garawyi. (2020). Phenotypic detection of AmpC ?-lactamase in Pseudomonas aerogenosa isolated from Burns and Wounds in Al-Rumetha Hospitals. Indian Journal of Forensic Medicine & Toxicology, 14(2), 790-797. https://doi.org/10.37506/ijfmt.v14i2.2960