Immunohistochemical Correlates of Epidermal Growth Factor Receptor Mutations in Lung Adenocarcinoma in Iraq Patient

Authors

  • Sabreen Saleem AbdAlkreem Alsaqi1, Merza Hamza Homady Almurshidi1, Rand Muhammed Abdul-Hussein Al-Husseini1

DOI:

https://doi.org/10.37506/ijfmt.v14i2.2968

Keywords:

Epidermal growth factor receptor (EGFR); Immunohistochemical; lung adenocarcacinoma; prognostic factors.

Abstract

We aimed to investigate the Detection of epidermal growth factor receptor (EGFR)mutations in lung adenocarcinoma by Immunohistochemical and correlation with Clinicopathological features. We used Fifty tissue blocks embedded in paraffin wax were obtained from 50 patient (16 female and 34males) suffering from lung adenocarcacinoma, while controls were 30 samples from healthy, Histopathological examination Study by (H and E stain)and detection of Mutation in EGFR(exon19) by RFLP-PCR, detection of protein expression EGFR by immunohistochemical method Our results have, confirmed the mean age of patients group was 51.84 +/-15.70 years and the median was 54, performed study the non correlation between stage of disease and age of patients. site of tumor was right sided of lung tumor, accounting for 58 % .Distribution of patients according to grade of tumor was, The results of this study showed non-significant correlation between age and grade, results of this study showed significant difference in EGFR Positive between patients and control healthy in Imunohistochemical, had found as not significant with age ,gender, sit of tumor, grade, stage, in Immunohistochemical method of EGFR Expression. while Gene EGFR deletion of exon 19were analyzed using mutant-enriched polymerase chain reaction (PCR) was not detected in all patients and control

Author Biography

Sabreen Saleem AbdAlkreem Alsaqi1, Merza Hamza Homady Almurshidi1, Rand Muhammed Abdul-Hussein Al-Husseini1

Department of Biology, Faculty of Science, University of Kufa/Iraq

Published

2020-04-29

How to Cite

Sabreen Saleem AbdAlkreem Alsaqi1, Merza Hamza Homady Almurshidi1, Rand Muhammed Abdul-Hussein Al-Husseini1. (2020). Immunohistochemical Correlates of Epidermal Growth Factor Receptor Mutations in Lung Adenocarcinoma in Iraq Patient. Indian Journal of Forensic Medicine & Toxicology, 14(2), 810-815. https://doi.org/10.37506/ijfmt.v14i2.2968