Multiplex PCR for detection of the predominant diarrheacausing protozoa (Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium parvum) in fecal samples of cattle from Babylon Province, Iraq
Keywords:Cattle, Cryptosporidium parvum, Entamoeba histolytica, feces, Giardia intestinalis, multiplex PCR, protozoa.
The work, here, was focused on the detection of the predominant diarrhea-causing protozoa (Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium parvum) in fecal samples of cattle from Babylon province, Iraq. Fifty fecal samples from 50 cattle were collected and processed using a specific multiplex polymerase chain reaction (mPCR) technique that employed the small subunit ribosomal RNA (SSrRNA) gene as a molecular target. Using specific primers for each protozoan, the results showed positive identification of the E. histolytica, G. intestinalis, and C. parvum in 38 (76%), 22 (44%), and 12 (24%), respectively, of the fecal samples. The data from the current investigation identify the presence of the E. histolytica, G. intestinalis, and C. parvum in the fecal samples collected from cattle located in Babylon province, Iraq.
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