Mitochondrial 16S rRNA gene-dependent Blood typing as a Forensic Tool
Keywords:Homo sapiens, Ovis aries, Capra hircus, Bos taurus, Forensic.
Background: Mitochondrial DNA is an important tool for human identification and is used to differentiate between human and animal blood at the crime scene, because in extreme conditions nuclear DNA is severely destroyed while Mitochondrial DNA contains multiple copies (200-2000) in per cell as well as resists harsh and more stable conditions. Methodology: Seventy-two blood samples were collected from human (Homo sapiens), sheep (Ovis aries), goat (Capra hircus) and cow (Bos taurus) (Eighteen blood samples for each). All blood samples were withdrawn by technician and 5ml were aspirated using aseptic technique and transferred to EDTA-Na2 tube and mixed well and stored in refrigerator. The collection takes 2 weeks (15th May 2019 to 30th May 2019). All samples were collected from Al-Diwanyia city. Results: The results of PCR reveal that, the primer pairs were specific and non-specific products not appear for all samples. The amplification of Homo sapiens mitochondrial DNA with primer pairs of other (Ovis aries, Capra hircus and Bos taurus) and amplification of each with primers pair of another genus gave negative results and this a primary evidence for primer pairs specificity. The amplicon of 16S rRNA gene of Homo sapiens were 1200bp ,Ovis aries were 1060bp ,Capra hircus were 820bp , and Bos taurus were 1300bp. The sequencing revealed that no crossreactivity of designed primer pairs and the PCR assay based on the designed primer pairs will be simple, fast, sensitive, specific, and cost-effective. Conclusion: Sensitivity, specificity and accuracy of the designed species specific primer pairs and applicability of the designed primer pairs in forensics to investigate blood sports or evidence belonging for human, sheep, goat and cow.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions