Macrophage Activity and Histopathological Differences of Lung Tissue on Sequential Co-infections of Heligmosomoides Polygyrus Nematode on Mycobacterium Tuberculosis Infection

Authors

  • Laksmi Wulandari1.2, Muhammad Amin1, Soedarto3, Gatot Soegiarto2.4, Kenji Ishiwata5

DOI:

https://doi.org/10.37506/ijfmt.v14i2.3181

Keywords:

Heligmosomoides polygyrus, Mycobacterium tuberculosis, macrophage, Immunohistochemistry

Abstract

Background: Tuberculosis is a chronic infection caused by Mycobacterium tuberculosis, a facultative intracellular parasite, that can be eliminated by cellular immunity played by macrophages. It has become a debate whether the co-infection of nematodes will affect the immune response of macrophages towards mycobacterium infection. Objective: To reveal macrophage activity and histopathological difference of lung tissue in sequential coinfection of Heligmosomoides Polygyrus towards Mycobacterium tuberculosis infection. Method: This study used 49 mice divided into 7 treatment groups with Mycobacterium tuberculose infection by inhalation and Heligmosomoides polygyrus orally within 8 and 16 weeks, and observed by immunohistochemical staining. Result: Infection for 8 weeks showed polarization of macrophages towards M1 macrophage, whereas in 16 weeks, the macrophage polarization more towards M2 macrophages, supported by histopathological changes of lung tissue: peribronchiolitis, perivaskulitis, alveolitis, and granuloma formation with counts of acid-resistant germs +3. There was a difference of expression of arginase1 to each group (p <0.001) and there was a difference of T CD4+ Th1 lymphocyte (p <0.001). Conclusion: There is a difference in macrophage activity in lung tissue; however, it does not cause different levels of histopathological changes in lung tissue and does not affect the immune response to Mycobacterium tuberculosis infection.

Author Biography

Laksmi Wulandari1.2, Muhammad Amin1, Soedarto3, Gatot Soegiarto2.4, Kenji Ishiwata5

1Department of Pulmonology and Respiratory, Faculty of Medicine, Universitas Airlangga, , Surabaya 60285, Indonesia, 2Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia, 3Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya 60285, Indonesia, 4Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya 60285, Indonesia, 5Department of Tropical Medicine, The Jikei University School of Medicine Tokyo 105-8461, Japan

Published

2020-04-29

How to Cite

Laksmi Wulandari1.2, Muhammad Amin1, Soedarto3, Gatot Soegiarto2.4, Kenji Ishiwata5. (2020). Macrophage Activity and Histopathological Differences of Lung Tissue on Sequential Co-infections of Heligmosomoides Polygyrus Nematode on Mycobacterium Tuberculosis Infection. Indian Journal of Forensic Medicine & Toxicology, 14(2), 1699-1704. https://doi.org/10.37506/ijfmt.v14i2.3181