Biocompatibility Test of Decellularized Cartilage Bovine Scaffold in Vitro and In Vivo


  • Achmad Fachrizal1, Dwikora Novembri Utomo1



DCBS, biocompatibility, In vivo, In vitro.


Background: Incidence of cartilage defects was reported at 65.00% of the routine arthroscopy procedures. Dr.Soetomo Teaching Hospital Network Bank developed a cartilage scaffold from bovine that has been deselularized decellularized cartilage bovine scaffold (DCBS). It takes a study of biocompatibility test to prove that DCBS is not cytotoxic to cells and not generate a host response. Objective: To analyze the biocompatibility of decellularized cartilage bovine scaffold (DCBS) in vivo and in vitro. Methods and Materials: The subjects were experimental rats and experimental with in vitro and in vivo trials. The subjects were treated by toxicity test with MTT assay, irritation test using Draize Scale, acute/ pyogenic systemic toxicity test by observing changes in body weight and temperature, and implantation test by observing fibrous capsule formation and Immunoglobulin G. Data was analyzed by using Kolmogorovsmirnov, Independent T-test, Mutivariate Analysis of Variance (MANOVA), and Mann Whitney. Result: The percentage of viable DCBS (81.78%) and CBS (92.45%) was ?70% of the control; thus, it is non-toxic to the cell. Draize scale in each group was in grade 0. Changes in body weight (p = 0.981) and temperature (p >0.05) had no significant association between groups. There was a significant difference mean of the fibrous capsule (p = 0.000) thickness and no significant difference in the mean number of Immunoglobulin G which was formed at week 1 (p = 0.87) and week 4 (p = 0.63) after implantation between CCBS and DCBS. Conclusion: The biocompatibility test shows that DCBS is compatible as a biomaterial.

Author Biography

Achmad Fachrizal1, Dwikora Novembri Utomo1

1Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya (60131), Indonesia



How to Cite

Achmad Fachrizal1, Dwikora Novembri Utomo1. (2020). Biocompatibility Test of Decellularized Cartilage Bovine Scaffold in Vitro and In Vivo. Indian Journal of Forensic Medicine & Toxicology, 14(2), 2313-2318.