Public Awareness for Antimicrobial Resistance from Escherichia coli Isolated from Beef Sold on Several Wet Market in Surabaya, Indonesia
Keywords:MDR, ESBL, Escherichia coli, Beef, Vitek-2 System, Public Health
Objective: This study aims to phenotypically identify and confirm the presence of multi-drug resistant
(MDR) and Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli in the swab surface
samples of beef using VITEK-2 method.
Materials and Methods: Swab samples were taken from five wet markets: Pucang market, Wonokromo
market, Keputran market, Pabean market, and Manukan market; 10 swab samples of beef were collected from
each market. Then, isolation and identification in terms of bacteria using selective media and biochemical
test were conducted. Resistance testing using disc diffusion method was performed on 6 types of antibiotics:
Ampicillin, Cefazolin, Ceftriaxone, Cefotaxime, Ceftazidime, Tetracyclin. Positive isolates resistant to ? 2
types of Beta-Lactam antibiotics using disc diffusion method then were tested using VITEK-2 method.
Results: Positive samples containing Escherichia coli are found in 29 samples out of 50 swab samples. Of
the 29 Escherichia coli isolates, 17 isolates are found resistant to the disc diffusion method. After testing
those 17 isolates using VITEK-2 method, 5% (1/17) of ESBL-producing Escherichia coli are obtained
which is resistant to all Beta-Lactam antibiotics. Besides, this study also reveals that 35% (6/17) of the
Escherichia coli are positive multidrug resistance. Those E.coli MDR are found to be resistant towards
antibiotic class Beta-Lactams, Aminoglycoside, Quinolon, and Sulfonamida.
Conclusion: This study has encouraged the need for public awareness for the understanding that beef
from wet market can be potential as reservoir to spread multi-drug resistant bacteria that can cause health
problems in humans.