Study the C/T Single Nucleotide Polymorphism at Tyrosine Kinase Domain of Insulin Receptor Gene in Patients with Polycystic Ovary Syndrome in Babylon Province
Keywords:Polycystic ovary syndrome, insulin resistance, insulin receptor, tyrosine kinase domain, His 1058 C/T.
Polycystic ovary syndrome (PCOS) is the commonest gynecological endocrinopathy, is characterized
by chronic anovulation and hyperandrogenism and is also associated with increase insulin secretion and
metabolic disease and insulin resistance. Present study aims to examine the association between the single
nucleotide polymorphism (rs1799817) of exon 17 of the insulin receptor (INSR) gene and polycystic ovary
syndrome (PCOS) in a Babylon women. Fifty women with PCOS and fifty healthy Iraqi women, age and
BMI matching were achieved between study groups. Biochemical parameters that estimated were fasting
glucose, fasting insulin, luteinizing hormone, testosterone. His 1058 C/T polymorphism at the tyrosine
kinase domain in the INSR gene was analyzed by restriction fragment length polymorphism (RFLPPCR). Significant increase in the levels of fasting glucose fasting insulin, HOMA-IR, LH, testosterone
were noticed in patient when compared with control. TT genotype frequency was higher in PCOS patients
whereas CC genotype was higher in control women. All parameters that estimate in this study were higher
in TT genotype of patient group. In conclusion there are an association of C/T polymorphism at His1058 of
INSR in patients women with PCOS in Babylon Province. Insulin resistance in PCOS patients might differ
from these that result from obesity.