The Effect of Fibrin Glue Application as a Sealant in Protecting Colon Anastomosis with Continues Suture Compared to Simple Interrupted Suture in Intraperitoneal Infection Condition Measured by Tissue Hydroxyproline Level (Wistar Rat Study)
Keywords:fibrin glue, simple interrupted suture, continuous suture, colon anastomosis, peritonitis
Background: Anastomotic leakage is a serious complication in gastrointestinal surgery, especially in
peritonitis condition. Simple interrupted suture is the gold standard for intestinal anastomosis; however, it
has a greater risk of contamination by peritoneal fluid. Fibrin glue has been used as a barrier to protect the
intestinal anastomotic suture by increasing hydroxyproline levels. But its benefit in continuous suture is still
Objective: To determine the effect of fibrin glue as a sealent in protecting colon anastomosis with continuous
suture compared to simple interrupted suture in intraperitoneal infection conditions.
Method: In this experimental study, the rats were divided into two groups: control group (Groups 1 and 2)
and experimental group (Groups 3 and 4). Group 1 and 3 were performed by simple interrupted, while the
others by continuous suture. Full-thickness incisions were made on the left colon and fibrin glue was applied
over the sutures in experimental groups. The samples were taken on day 10.
Result: According to hydroxyproline levels (mcg/gr tissue), average values of the groups was 201.18 ±
27.81 (Group 1), 208.73 ± 17.81 (Group 2), 245.83 ± 20.47 (Group 3), and 241.55 ± 16.85 (Group 4). Tissue
hydroxyproline levels in experimental groups were significantly higher compared to control (P < 0.05). In
experimental group, mean hydroxyproline levels in subject with continuous suture was lower than simple
interrupted suture; however it was not significant statistically (p=0.796).
Conclusion: As a result, the use of fibrin glue is beneficial to increase hydroxyproline level in colon
anastomotic tissue especially in simple interrupted than continuous suture technique.
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