The Effect of Fibrin Glue Application as a Sealant in Protecting Colon Anastomosis with Continues Suture Compared to Simple Interrupted Suture in Intraperitoneal Infection Condition Measured by Tissue Hydroxyproline Level (Wistar Rat Study)

Authors

  • Agung Fuad Fathurochman1, Vicky Sumarki Budipramana2, Jusak Nugraha3

DOI:

https://doi.org/10.37506/ijphrd.v12i1.13848

Keywords:

fibrin glue, simple interrupted suture, continuous suture, colon anastomosis, peritonitis

Abstract

Background: Anastomotic leakage is a serious complication in gastrointestinal surgery, especially in
peritonitis condition. Simple interrupted suture is the gold standard for intestinal anastomosis; however, it
has a greater risk of contamination by peritoneal fluid. Fibrin glue has been used as a barrier to protect the
intestinal anastomotic suture by increasing hydroxyproline levels. But its benefit in continuous suture is still
debatable.
Objective: To determine the effect of fibrin glue as a sealent in protecting colon anastomosis with continuous
suture compared to simple interrupted suture in intraperitoneal infection conditions.
Method: In this experimental study, the rats were divided into two groups: control group (Groups 1 and 2)
and experimental group (Groups 3 and 4). Group 1 and 3 were performed by simple interrupted, while the
others by continuous suture. Full-thickness incisions were made on the left colon and fibrin glue was applied
over the sutures in experimental groups. The samples were taken on day 10.
Result: According to hydroxyproline levels (mcg/gr tissue), average values of the groups was 201.18 ±
27.81 (Group 1), 208.73 ± 17.81 (Group 2), 245.83 ± 20.47 (Group 3), and 241.55 ± 16.85 (Group 4). Tissue
hydroxyproline levels in experimental groups were significantly higher compared to control (P < 0.05). In
experimental group, mean hydroxyproline levels in subject with continuous suture was lower than simple
interrupted suture; however it was not significant statistically (p=0.796).
Conclusion: As a result, the use of fibrin glue is beneficial to increase hydroxyproline level in colon
anastomotic tissue especially in simple interrupted than continuous suture technique.

Author Biography

Agung Fuad Fathurochman1, Vicky Sumarki Budipramana2, Jusak Nugraha3

1
Resident of Surgery, Faculty of Medicine Airlangga University - Soetomo General Hospital Surabaya, 2Staff of
Digestive Surgery Division, Department of Surgery, Faculty of Medicine Airlangga University - Soetomo General
Hospital Surabaya, 3Staff of Department of Clinical Pathology, Faculty of Medicine Airlangga University -
Soetomo General Hospital Surabaya

Published

2021-01-07

How to Cite

Agung Fuad Fathurochman1, Vicky Sumarki Budipramana2, Jusak Nugraha3. (2021). The Effect of Fibrin Glue Application as a Sealant in Protecting Colon Anastomosis with Continues Suture Compared to Simple Interrupted Suture in Intraperitoneal Infection Condition Measured by Tissue Hydroxyproline Level (Wistar Rat Study). Indian Journal of Public Health Research & Development, 12(1), 191-197. https://doi.org/10.37506/ijphrd.v12i1.13848