In Silico analysis of Phospho Enolpyrovate Carboxylase gene
Keywords:RNA isolation, (PEPcase) analysis, sugar production, sequencing.
The importance of sugar in many aspects of life, such as food, health and body care products, results in
its great demand all around the world. From another hand, the annual production of sugar cannot meet the
increasing demand of the growing human population. Therefore, this study focused on one of the most
important genes that affect the quantity of sugar production, phosphoenolpyruvate carboxylase (PEPcase).
The most important crop used to extract sugar is sugarcane where sugar is stored in the stalk internodes. In
this study RNA extraction was performed to produce cDNA for cloning. Sequencing the main genes using
the Basic Local Alignment Search Tool (BLAST).
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