Antimicrobial and Biofilm Inhibitory Activity of Nanoparticles Against Clinical Isolates from Urinary Tract Infection

Abstract

At these days nanoparticles (NPs) are used as antibacterial due to its have chemical-physical addition to

biological effect. A big group of microbial cells adhering to a surface are called biofilm. Exposure to nano

particles such as (Ag, Al2O3, Ni) may prevent colonization of new bacteria onto the biofilm. In the present

research we have analyze whether the biofilm formation of some isolates of pathogenic bacteria could be

influenced by NPs. Also we examined the susceptibility of the isolates to some antibiotics in combination

with Ag, Al2O3 and Ni nanoparticles. (50) Strains of isolated K. pneumonia have been examine from patients

with urinary tract infection were collected from Education of Baghdad hospitals in Iraq. Serial dilution of

Tube method to determination of MIC and MBEC for Nanoparticles against isolates was done. Biofilm

formation was evaluated by micro titer plates. Additionally. disc diffusion method was used to assay the

various antibiotics and combinations for bactericidal activity against the isolates. The results showed that

about 97% of the strains were capable to form of structure biofilms. More than 82% about of strains showed

resistance to commonly used antibiotics. Antibiotics and antimicrobials from mixed nanoparticles were

higher than compared to nanoparticles alone. Therefore, a synergistic effect was observed between silver -

Nickel nanoparticles and silver - aluminum oxide nanoparticles in to inhibiting the growth of K. pneumonia

strains. Furthermore, cytotoxicity activity of Ag NPs against Neuro-2a cells was higher than Al2O3 NPs. The

results suggest that nanoparticles can be used as antimicrobial agents for therapeutic to treat urinary tract

infections caused by K. pneumoniae.