Molecular Detection blaOXA50 gene of P.aerginosae Isolated from Otitis Media
Keywords:P.aerginosae, blaOXA50 gene, Resistance antibiotic
Pseudomonas aerugenosa is a gram negative rod shape bacterium belonging to the family Pseudomonadaceae
. Pseudomonas aeruginosa is a major uropathogen in a hospital. It can tolerate a wide kind of physical
conditions and many antibiotics via several mechanisms resistance . The study included genetic study
blaOXA50 gene utilizer technique PCR by specific forward and reverse primers to common bacteria that
causes otitis media, 40 isolated from otitis media patients (ear swab). Primary identification was depended
on Gram stain ,biochemical tests and vitek 2 system was done . The results demonstrate 30 isolate of them
were P.aerginosae. The result appear P.aerginosae contain on 25(19%) blaOXA50 gene.
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